Analytical and Experimental study of Liquid chromatography-mass spectrometry (LC-MS) profiling of the dehydrated Allium sativum and Allium cepa extracts; a gateway to therapeutic drug development.

Q2 Medicine

Clinica Terapeutica Pub Date : 2024-09-01 DOI:10.7417/CT.2024.5131

N Shyamala, S Naresh, A K Nayanatara, H Anupama, D Shivananda

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引用次数: 0

Abstract

Purpose: Practice of medicinal plants for the treatment of various ailments is a history for several decades. Occurrence of active compounds in these plants makes it significant in the era of modern medicine. Garlic and onion a monocotyledonous bulbous flowering plant belonging to the genus Allium has an extensive tradition for being used as anti-inflammatory, anti-cancer, anti-hyperepidemic, anti-diabetic, anti-bacterial, neuroprotective agent against various disorders. Modern day synthetic formulations have reduced efficacy and rendered with side effects that channelled the exploration for alternate source in plants for dealing with various disorders. The present study aims to explore the bioactive compounds present in Allium sativum (Garlic) and Allium cepa (Red Onion).

Methods: Garlic and onion powder was soaked in Methanol, filtered, and concentrated in a vacuum in a rotary evaporator. Fractionation was carried out.LC-MS analysis of aqueous fraction was performed using a Waters Mass Q-TOF Mass Spectrometer with diode array detector.

Results: LC-MS analysis revealed the occurrence of 200 compounds in garlic and 184 compounds in onion. 3.53% in garlic and 4.37% in onion were the major chemical constituents based on the number of hits.

Conclusion: This study suggests that the Allium sativum and Allium cepa herbal powders being loaded with large number of active compounds would be promising alternative agents due to their richness in antioxidants.

查看原文本刊更多论文

脱水薤白和牛肝菌提取物的液相色谱-质谱（LC-MS）分析和实验研究；治疗药物开发的途径。

目的：利用药用植物治疗各种疾病已有几十年的历史。这些植物中含有的活性化合物使其在现代医学时代具有重要意义。大蒜和洋葱是属于葱属的单子叶球根开花植物，具有广泛的传统，可用作抗炎、抗癌、抗高血压、抗糖尿病、抗菌和神经保护剂，以治疗各种疾病。现代合成制剂的药效降低，且有副作用，因此人们开始从植物中寻找治疗各种疾病的替代来源。本研究旨在探索大蒜（Allium sativum）和红洋葱（Allium cepa）中的生物活性化合物：方法：将大蒜和洋葱粉末浸泡在甲醇中，过滤后在旋转蒸发仪中真空浓缩。使用带二极管阵列检测器的 Waters Mass Q-TOF 质谱仪对水馏分进行 LC-MS 分析：结果：LC-MS 分析显示大蒜中含有 200 种化合物，洋葱中含有 184 种化合物。根据命中数量，大蒜中 3.53% 的化合物和洋葱中 4.37% 的化合物是主要化学成分：这项研究表明，大蒜和洋葱草药粉富含大量活性化合物，是很有前途的替代药物，因为它们含有丰富的抗氧化剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinica Terapeutica PHARMACOLOGY & PHARMACY-

CiteScore

2.50

自引率

0.00%

发文量

124

审稿时长

6-12 weeks

期刊介绍： La Clinica Terapeutica è una rivista di Clinica e Terapia in Medicina e Chirurgia, fondata nel 1951 dal Prof. Mariano Messini (1901-1980), Direttore dell''Istituto di Idrologia Medica dell''Università di Roma “La Sapienza”. La rivista è pubblicata come “periodico bimestrale” dalla Società Editrice Universo, casa editrice fondata nel 1945 dal Comm. Luigi Pellino. La Clinica Terapeutica è indicizzata su MEDLINE, INDEX MEDICUS, EMBASE/Excerpta Medica.