{"title":"Developmental characteristics of cutaneous telocytes in late embryos of the silky fowl.","authors":"Hao Li, Junliang Chen, Wenjun You, Yizhen Xu, Yaqiong Ye, Haiquan Zhao, Junxing Li, Hui Zhang","doi":"10.4081/ejh.2024.4089","DOIUrl":null,"url":null,"abstract":"<p><p>Telocytes (TCs) have been identified in various animals. However, information on TCs in the embryos is still very limited. In this work, the developing skin of the silky fowl was sampled for TCs identification by histology, immunohistochemistry and transmission electron microscopy. In addition, morphological parameters of cutaneous TCs and their location relationships were measured using a morphometry software - ImageJ (FiJi). At the 12th, 16th and 20th day of incubation, in the embryonic skin, telocyte-like cells (TC-L) were observed in the dermis. TCs were PDGFRα+ at the 12th, 16th and 20th day of incubation, but showed CD34+ only at 20th day of incubation in the embryonic dermis. Ultrastructurally, TCs were observed in the dermis at all late embryonic developmental stages. TCs established the homocellular contacts/plasmalemmal adhesion with each other. TCs established heterocellular contacts with melanocytes at 20th day of incubation in the dermis. In addition, the intracellular microvesicles were present in the cytoplasm of TCs. The extracellular microvesicles/exosomes were in close proximity to the TCs. The results confirmed that the locations, immunophenotypes, structural characteristics and relationships of TCs, and revealed the developmental characteristics of cutaneous TCs in late silky fowl embryos.</p>","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 4","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532996/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Histochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4081/ejh.2024.4089","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Telocytes (TCs) have been identified in various animals. However, information on TCs in the embryos is still very limited. In this work, the developing skin of the silky fowl was sampled for TCs identification by histology, immunohistochemistry and transmission electron microscopy. In addition, morphological parameters of cutaneous TCs and their location relationships were measured using a morphometry software - ImageJ (FiJi). At the 12th, 16th and 20th day of incubation, in the embryonic skin, telocyte-like cells (TC-L) were observed in the dermis. TCs were PDGFRα+ at the 12th, 16th and 20th day of incubation, but showed CD34+ only at 20th day of incubation in the embryonic dermis. Ultrastructurally, TCs were observed in the dermis at all late embryonic developmental stages. TCs established the homocellular contacts/plasmalemmal adhesion with each other. TCs established heterocellular contacts with melanocytes at 20th day of incubation in the dermis. In addition, the intracellular microvesicles were present in the cytoplasm of TCs. The extracellular microvesicles/exosomes were in close proximity to the TCs. The results confirmed that the locations, immunophenotypes, structural characteristics and relationships of TCs, and revealed the developmental characteristics of cutaneous TCs in late silky fowl embryos.
期刊介绍：
The Journal publishes original papers concerning investigations by histochemical and immunohistochemical methods, and performed with the aid of light, super-resolution and electron microscopy, cytometry and imaging techniques. Coverage extends to:
functional cell and tissue biology in animals and plants;
cell differentiation and death;
cell-cell interaction and molecular trafficking;
biology of cell development and senescence;
nerve and muscle cell biology;
cellular basis of diseases.
The histochemical approach is nowadays essentially aimed at locating molecules in the very place where they exert their biological roles, and at describing dynamically specific chemical activities in living cells. Basic research on cell functional organization is essential for understanding the mechanisms underlying major biological processes such as differentiation, the control of tissue homeostasis, and the regulation of normal and tumor cell growth. Even more than in the past, the European Journal of Histochemistry, as a journal of functional cytology, represents the venue where cell scientists may present and discuss their original results, technical improvements and theories.
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