Prevalence and diversity of spotted fever group Rickettsia species in ixodid ticks from domestic dogs in Chad, Africa

IF 3.1 2区医学 Q2 INFECTIOUS DISEASES

Ticks and Tick-borne Diseases Pub Date : 2024-10-19 DOI:10.1016/j.ttbdis.2024.102405

Stephanie Osip , Morgan Friedman , Ellen Haynes , Sarah M. Coker , John A. Bryan II , Metinou Sidouin , Philip Tchindebet Ouakou , Bongo Nare Richard Ngandolo , Christopher A. Cleveland , Michael J. Yabsley

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引用次数: 0

Abstract

Tick-borne pathogens in the genus Rickettsia are the causative agents of severe and potentially fatal spotted fever group (SFG) and typhus group diseases in dogs and humans. Climate, habitat, and land-use changes are impacting vector ranges, with expansions potentially resulting in novel pathogens being introduced into naïve locations. Despite the public health importance of SFG Rickettsia, there are relatively few data on the prevalence and diversity of rickettsial pathogens in sub-Saharan Africa. The aim of this study was to characterize the SFG Rickettsia prevalence and diversity in ixodid ticks (104 Amblyomma spp., 160 Rhipicephalus spp., and one Hyalomma truncatum) collected from domestic dogs in Chad, Africa. Ticks were screened for Rickettsia spp. using a nested PCR targeting the 17-kDa gene. Species identification was through bidirectional Sanger sequencing of the 17-kDa, ompA, ompB, and/or gltA gene targets. A total of 43.3 % (115/265) ticks were positive for Rickettsia spp. and six Rickettsia species were identified: R. africae, R. massiliae, R. conorii, R. felis, R. monacensis and Candidatus Rickettsia muridii. Seven additional samples were positive for Rickettsia of undetermined species. Rickettsia africae, an important zoonotic pathogen, was found in 81 % (79/97) of A. variegatum and 29 % (2/7) of an A. marmoreum complex species, a group that infests a wide range of birds and mammals, including humans. Finally, we detected a high diversity of Rickettsia spp., most of which were zoonotic, in Rh. muhsamae. Collectively these data indicate there is a risk of rickettsiosis in Chad and further studies on ticks and rickettsial pathogens in this region are warranted.

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非洲乍得家犬身上的伊科蜱中斑疹热立克次体的流行率和多样性。

立克次体属蜱媒病原体是狗和人类严重且可能致命的斑疹热类群（SFG）和斑疹伤寒类群疾病的病原体。气候、栖息地和土地使用的变化正在影响病媒的分布范围，病媒分布范围的扩大有可能导致新型病原体被引入新的地区。尽管SFG立克次体对公共卫生具有重要意义，但有关撒哈拉以南非洲地区立克次体病原体流行和多样性的数据却相对较少。本研究的目的是描述从非洲乍得家犬身上采集的伊科蜱（104只Amblyomma属、160只Rhipicephalus属和1只Hyalomma truncatum）中SFG立克次体的流行率和多样性。利用针对 17-kDa 基因的巢式 PCR 对蜱进行了立克次体筛选。通过对 17-kDa、ombA、ombB 和/或 gltA 基因靶标进行双向 Sanger 测序来鉴定物种。共有 43.3 %（115/265）的蜱对立克次体属呈阳性，并确定了 6 个立克次体种：R. africae、R. massiliae、R. conorii、R. felis、R. monacensis 和 Candidatus Rickettsia muridii。另有 7 份样本对未确定种类的立克次体呈阳性反应。在81%（79/97）的A. variegatum和29%（2/7）的A. marmoreum复合种中发现了非洲立克次体，这是一种重要的人畜共患病原体，可侵染包括人类在内的多种鸟类和哺乳动物。最后，我们在 Rh. muhsamae 中检测到了立克次体属的高度多样性，其中大部分是人畜共患的立克次体属。总之，这些数据表明乍得存在立克次体病的风险，因此有必要对该地区的蜱虫和立克次体病原体进行进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.