The Effect of C-X-C Motif Chemokine Ligand 12 in Colorectal Cancer Associated with Chemoresistance and Radioresistance as Well as Stemness.

IF 1.3 4区医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Iranian Journal of Public Health Pub Date : 2024-09-01 DOI:10.18502/ijph.v53i9.16461

Wuzhen Dong, Wen Lin, Chong Li

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引用次数: 0

Abstract

Background: We aimed to explore the role of C-X-C motif chemokine ligand 12 (CXCL12) and cytokinecytokine receptor interaction signaling pathway in the radiotherapy and chemotherapy resistance as well as cell stemness in colorectal cancer (CRC).

Methods: Bioinformatics analysis was used to identify the differentially expressed mRNAs and signal pathways closely related to differentially expressed mRNAs have also been analyzed in March 2022 at the Jinhua Central Hospital, China. Then, the expression of CXCL12 was detected by qRT-PCR in colorectal cancer cells and testing the effects of transfecting CXCL12 into different CRC-derived cell lines. The effects of CXCL12 on cell proliferation were evaluated by chemosensitivity assay and radiation sensitivity assay.

Results: Bioinformatics analysis of DEGs found a total of 2429 differentially expressed genes, THBS3 and CXCL12 genes are two abnormally highly expressed genes in the CRC. KEGG analysis showed the correlative signaling pathway, cytokine-cytokine receptor interaction, which is related to cell stemness. Furthermore, the expression of CXCL12 in CRC cells was detected and an increasing trend was obtained in CRC cells. In addition, the chemosensitivity and radiotherapy tolerance were elevated after transfected with CXCL12.

Conclusion: CXCL12 could be a potential promote biomarkers in CRC and also promote the chemosensitivity and radiotherapy tolerance.

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C-X-C Motif Chemokine Ligand 12 在结直肠癌中的作用与化疗耐药性、放射耐药性和干性有关

研究背景目的：探讨C-X-C位点趋化因子配体12（CXCL12）和细胞因子受体相互作用信号通路在大肠癌（CRC）放化疗耐药和细胞干性中的作用：方法：采用生物信息学分析方法确定差异表达的mRNA，并分析了与差异表达mRNA密切相关的信号通路。然后，通过qRT-PCR检测CXCL12在结直肠癌细胞中的表达，并测试将CXCL12转染到不同的CRC衍生细胞系中的效果。CXCL12对细胞增殖的影响通过化学敏感性实验和辐射敏感性实验进行评估：对 DEGs 的生物信息学分析发现，共有 2429 个差异表达基因，THBS3 和 CXCL12 基因是 CRC 中两个异常高表达的基因。KEGG分析显示，细胞因子-细胞因子受体相互作用是与细胞干性相关的信号通路。此外，还检测到 CXCL12 在 CRC 细胞中的表达，并发现其在 CRC 细胞中呈上升趋势。此外，转染 CXCL12 后，化疗敏感性和放疗耐受性均有所提高：结论：CXCL12 是一种潜在的促进 CRC 生长的生物标记物，它还能提高 CRC 的化疗敏感性和放疗耐受性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

2.20

自引率

7.10%

发文量

300

审稿时长

3-8 weeks

期刊介绍： Iranian Journal of Public Health has been continuously published since 1971, as the only Journal in all health domains, with wide distribution (including WHO in Geneva and Cairo) in two languages (English and Persian). From 2001 issue, the Journal is published only in English language. During the last 41 years more than 2000 scientific research papers, results of health activities, surveys and services, have been published in this Journal. To meet the increasing demand of respected researchers, as of January 2012, the Journal is published monthly. I wish this will assist to promote the level of global knowledge. The main topics that the Journal would welcome are: Bioethics, Disaster and Health, Entomology, Epidemiology, Health and Environment, Health Economics, Health Services, Immunology, Medical Genetics, Mental Health, Microbiology, Nutrition and Food Safety, Occupational Health, Oral Health. We would be very delighted to receive your Original papers, Review Articles, Short communications, Case reports and Scientific Letters to the Editor on the above mentioned research areas.