Mehdi Salar Amoli, Linqi Jin, Sarah Rezapourdamanab, Maher Saadeh, Yamini Singh, Liqun Ning, Boeun Hwang, Martin L Tomov, Christopher N LaRock, Morteza Mahmoudi, Holly Bauser-Heaton, Vahid Serpooshan
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引用次数: 0
Abstract
This protocol describes the preparation of a nanoparticle-encapsulated bioink capable of protecting tissue-engineered constructs against bacterial infections while also providing contrast for magnetic resonance (MR) imaging modalities. The report includes details of preparing the methacrylated gelatin-based bioinks and the incorporation of superparamagnetic iron oxide nanoparticles. A detailed protocol is presented for characterizing the bioink, evaluating cell response, and assessing its antibacterial effect. Overall, this article presents a robust approach for the development of antibacterial, MR-visible bioinks suitable for various tissue engineering applications. For complete details on the use and execution of this protocol, please refer to Theus et al.1.
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