Genomic characterization and survey of a second luteovirus infecting blueberries

IF 2.5 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2024-10-26 DOI:10.1016/j.virusres.2024.199480

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引用次数: 0

Abstract

New and emerging viral problems may be contributing to blueberry decline. In this research we described a new virus detected in Oregon blueberry production field and surveyed the region for its potential spread. The complete genome sequence of a putative new member of the genus Luteovirus was obtained from blueberry (Vaccinium corymbosum L.) by high throughput sequencing and 5′/3′-RACE. The new virus was tentatively named blueberry virus M (BlVM). Its genome is 5,018 nt long with four putative open reading frames. Similarly to some recently discovered luteoviruses, BlVM does not possess any movement protein (MP). Phylogenetic analysis confirmed clustering of BlVM with the group of non-MP luteoviruses, showing blueberry virus L as the most similar species. Through a small-scale high throughput sequencing survey we obtained 14 additional near complete genomic sequences. A larger survey of 2,654 samples by RT-PCR in Oregon and Washington (USA) found 52 BlVM-positive plants collected from four locations in Oregon. These findings will facilitate monitoring virus distribution and assessment of potential disease associated with this new and emerging blueberry virus.

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感染蓝莓的第二种黄体病毒的基因组特征和调查。

新出现的病毒问题可能会导致蓝莓产量下降。在这项研究中，我们描述了在俄勒冈州蓝莓产地发现的一种新病毒，并调查了该地区的潜在传播情况。通过高通量测序和 5'/3'-RACE 技术，我们从蓝莓（Vaccinium corymbosum L.）中获得了一种假定的 Luteovirus 属新成员的完整基因组序列。新病毒被暂时命名为蓝莓病毒 M（BlVM）。它的基因组长 5,018 nt，有四个推测的开放阅读框。与最近发现的一些黄体病毒类似，BlVM不具有任何运动蛋白（MP）。系统进化分析证实，BlVM 与无运动蛋白的黄体病毒群聚类，显示蓝莓病毒 L（BlVL）是最相似的物种。通过小规模的高通量测序调查，我们又获得了 14 个接近完整的基因组序列。通过 RT-PCR 技术对美国俄勒冈州和华盛顿州的 2,654 份样本进行了更大规模的调查，发现从俄勒冈州的四个地点采集的 52 株 BlVM 阳性植株。这些发现将有助于监测病毒的分布情况，并评估与这种新出现的蓝莓病毒相关的潜在疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.