Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14636

Silvia Gimeno-Martos, Luigia Bosa, Pedro Luis Lorenzo, María Arias-Alvarez, Cesare Castellini, Pilar García-Rebollar, Rosa María García-García

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引用次数: 0

Abstract

β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination.

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壳聚糖游离和微囊化重组兔神经生长因子对兔精子质量参数的影响

β-神经生长因子（βNGF）在生殖生理和精子质量中起着至关重要的作用。精浆和阴道液中的酶活性会减少可用的 βNGF，有研究表明壳聚糖微球可以保护 rrβNGF 免受降解。本研究考察了壳聚糖微囊化 rrbNGF 对家兔精子活力、运动和获能状态的影响。结果表明，0.5 和 1 μg/mL 的微囊化 rrβNGF 以及游离 rrβNGF 或空微球在孵育 2 小时后不会对精子活力或总运动能力产生不利影响。然而，1 μg/mL游离rrβNGF可观察到最高的渐进动力学参数，而0.5 μg/mL微囊化rrβNGF可观察到最高的曲线速度（VCL）。空壳聚糖微球不会诱导顶体反应（AR），但在体外培养过程中，两种浓度的游离和rrβNGFch都有利于顶体反应。该研究表明，使用壳聚糖球不会对精子的性状产生任何不利影响，而游离rβNGF和rrβNGFch则会促进获能和AR。需要进一步研究 rrβNGFch 在人工授精过程中改变体外获能和诱导排卵的潜力。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.