Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14594

Mònica Ferrer-Roda, Jaime Catalán, Dolors Izquierdo, Maria Emilia Franco Oliveira, Maria-Teresa Paramio

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引用次数: 0

Abstract

Goat production is affected by reproductive seasonality. In vitro embryo production (IVEP) could overcome this effect. This study aimed to evaluate the impact of the season of semen collection/freezing on IVEP of prepubertal goat oocytes and on sperm quality and functionality concerning capacitation. Semen from six fertile bucks was collected, pooled and cryopreserved in spring and autumn and used for IVEP of oocytes recovered during the breeding season. Oocytes were IVM in TCM-199 with hormones, EGF and cysteamine; fertilized and cultured in BO-IVF and BO-IVC media (IVF Bioscience, UK). Semen samples were assessed at 0 and 3 h after culture in capacitating (BO-IVF, CAP) and non-capacitating conditions for sperm plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), intracellular calcium and plasma membrane lipid disorder. Blastocyst production was higher with spring sperm compared to autumn (12.0% vs. 2.1%, respectively; p < .05). After CAP, acrosome reaction and intracellular calcium were higher (p < .05) in spring than autumn sperm. No differences were found in other sperm parameters. In conclusion, seasonal variations in the IVEP of prepubertal goats could be linked to differences in sperm ability to undergo in vitro capacitation.

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精液冷冻季节对青春期前山羊卵母细胞体外胚胎生产的影响。

山羊生产受繁殖季节性的影响。体外胚胎生产（IVEP）可以克服这种影响。本研究旨在评估精液采集/冷冻季节对青春期前山羊卵母细胞体外受精的影响，以及对精子质量和获能功能的影响。研究人员在春季和秋季收集、汇集和冷冻保存了六头可育公羊的精液，并将其用于繁殖季节回收的卵母细胞的IVEP。卵母细胞在含有激素、EGF和半胱胺的TCM-199中进行体外受精；在BO-IVF和BO-IVC培养基（IVF Bioscience，英国）中受精和培养。精液样本在获能（BO-IVF，CAP）和非获能条件下培养 0 和 3 小时后进行精子质膜和顶体完整性、线粒体膜电位（MMP）、细胞内钙和质膜脂质紊乱的评估。与秋季精子相比，春季精子的囊胚产量更高（分别为 12.0% 和 2.1%；p

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.