High cooling rate of 60°C/min around ice nucleation during cryopreservation compromises chicken sperm viability.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14626

Adolfo Toledano-Díaz, Rosalina Robledo, Esther Alba, Pablo Fernández-Alaez, Pedro García-Casado, Berenice Bernal, Cristina Castaño, Olga Torres, Wendy M Rauw, Julián Santiago-Moreno

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引用次数: 0

Abstract

The present study compares two protocols for the cryopreservation of chicken semen. Both protocols had an initial low cooling rate in the first step, followed by higher cooling rates around ice nucleation (Protocol 1) or following the dissipation of the latent heat of fusion (Protocol 2) in the second step. Semen ejaculates obtained from 12 roosters were diluted with Rootex with 6% dimethylformamide and frozen following either Protocol 1 (from +5°C to -10°C at 5°C/min and from -10°C to -130°C at 60°C/min) or Protocol 2 (from +5°C to -35°C at 7°C/min and from -35°C to -140°C at 60°C/min). Compared with fresh semen, following both protocols, cryopreservation resulted in reduced post-thaw sperm quality (p < .001). Post-thaw percentage of sperm with an intact plasma membrane was greater using Protocol 2 than Protocol 1 (p < .05). The results suggest that high cooling rates around the time of ice nucleation are not recommendable.

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在冷冻保存过程中，冰核周围 60°C/min 的高冷却速度会影响鸡精子的存活率。

本研究比较了两种鸡精液冷冻保存方案。两种方案在第一步中的初始冷却速率都较低，然后在第二步中，在冰核形成（方案 1）或融合潜热消散（方案 2）前后采用较高的冷却速率。将 12 只公鸡的精液用含有 6% 二甲基甲酰胺的 Rootex 稀释，然后按照步骤 1（从 +5°C 到 -10°C，5°C/分钟，从 -10°C 到 -130°C ，60°C/分钟）或步骤 2（从 +5°C 到 -35°C，7°C/分钟，从 -35°C 到 -140°C ，60°C/分钟）进行冷冻。与新鲜精液相比，采用这两种方案后，冷冻保存会导致解冻后精子质量下降（p

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.