A short duration of mechanical ventilation alters redox status in the diaphragm and aggravates inflammation in septic mice

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-19 DOI:10.1016/j.resp.2024.104361

Pedro Alves Machado-Junior , Marcelo Santiago Soares Dias , Ana Beatriz Farias de Souza , Leonardo Spinelli Estevão Lopes , Tatiana Prata Menezes , André Talvani , Laurent Brochard , Frank Silva Bezerra

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引用次数: 0

Abstract

Background

Mechanical ventilation (MV) is a life support method used to treat patients with respiratory failure. High tidal volumes during MV can cause ventilator-induced lung injury (VILI), but also affect other organs, such as the diaphragm (Dia) causing ventilator-induced diaphragmatic dysfunction (VIDD). VIDD is often associated with a complicated course on MV. Sepsis can induce inflammation and oxidative stress, contributing to the impairment of the Dia and worsening of the prognosis. This study evaluated the additive or synergistic effects of a short course of mechanical ventilation on Dia in healthy and septic adult mice.

Methods

32 adult male C57BL/6 mice were randomly into four groups: Control (CG), non-ventilated animals instilled with saline solution (PBS1x); Lipopolysaccharide (LPS), non-ventilated animals instilled with PBS solution containing lipopolysaccharide; Mechanical Ventilation (MV) for 1 h, ventilated animals instilled with PBS solution; and Mechanical Ventilation and LPS (MV+LPS), ventilated animals instilled with PBS solution containing LPS. At the end of the experimental protocol, the animals were euthanized, then blood and diaphragm tissue samples were collected.

Results

Evaluation of leukocyte/blood parameters and diaphragm muscle showed that MV, LPS and the combination of both were able to increase neutrophil count, creatine kinase, inflammatory mediators and oxidative stress in all groups compared to the control. MV and sepsis combined had additive effects on inflammation and lipid peroxidation.

Conclusions

A short course of Mechanical ventilation promotes inflammation and oxidative stress and, its combination with sepsis further increases local and systemic inflammation.

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短时间的机械通气会改变膈肌的氧化还原状态，加重脓毒症小鼠的炎症。

背景：机械通气（MV）是一种用于治疗呼吸衰竭患者的生命支持方法。机械通气期间的高潮气量可导致呼吸机诱发肺损伤（VILI），但也会影响其他器官，如引起呼吸机诱发膈肌功能障碍（VIDD）的膈肌（Dia）。VIDD 通常与 MV 的复杂病程有关。脓毒症可诱发炎症和氧化应激，导致膈肌受损和预后恶化。本研究评估了短期机械通气对健康和脓毒症成年小鼠 Dia 的叠加或协同作用：方法：将32只成年雄性C57BL/6小鼠随机分为四组：对照组（CG），灌注生理盐水（PBS1x）的非通气动物；脂多糖组（LPS），灌注含有脂多糖的PBS溶液的非通气动物；机械通气1小时组（MV），灌注PBS溶液的通气动物；机械通气和LPS组（MV+LPS），灌注含有LPS的PBS溶液的通气动物。实验结束后，对动物实施安乐术，然后采集血液和膈肌组织样本：结果：对白细胞/血液参数和膈肌的评估表明，与对照组相比，中性粒细胞增多症、脓毒症和两者的结合能增加所有组的中性粒细胞计数、肌酸激酶、炎症介质和氧化应激。中压和脓毒症联合作用对炎症和脂质过氧化具有叠加效应：结论：短期机械通气会促进炎症和氧化应激，与败血症合并使用会进一步增加局部和全身炎症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.