Prolonged incubation of frozen-thawed equine spermatozoa for in vitro fertilization: A preliminary study using low temperature and INRA96 medium.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14593

Marcos Luis-Calero, Carmen C Muñoz-García, Pablo Fernández-Hernández, Beatriz Macías-García, Lauro González-Fernández

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Abstract

A protocol for conventional in vitro fertilization (IVF) in horses using fresh semen has been described, using a prolonged incubation in FERT-TALP medium (22 h) at 38.2°C in the presence of penicillamine, hypotaurine and epinephrine (PHE). Our work aimed to develop a protocol that maintains quality parameters in frozen-thawed equine spermatozoa incubated for 22 h in the presence of PHE using different media (FERT-TALP and INRA96) and incubation temperatures (30 and 38.2°C). Twelve frozen ejaculates from four stallions were thawed and then incubated in either FERT-TALP or INRA96 with PHE at 30 or 38.2°C for 22 h. Following incubation, total motility (TM), progressive motility (PM), viability and acrosome integrity were evaluated. The results showed that TM was significantly higher (p < .001) at 30°C in both media, while PM was higher for INRA96 at 30°C compared to 38°C (p < .05). Moreover, INRA96 at 30°C exhibited higher sperm viability and acrosome integrity (p < .001) compared to the other experimental groups. These preliminary results suggest that incubating thawed equine spermatozoa at 30°C with PHE in INRA96 successfully maintains motility, viability and acrosome integrity in equine spermatozoa, indicating its potential use for conventional equine IVF.

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用于体外受精的冷冻解冻马精子的长时间培养：使用低温和 INRA96 培养基的初步研究。

有一种使用新鲜精液对马进行常规体外受精（IVF）的方案，即在青霉胺、低牛磺酸和肾上腺素（PHE）作用下，在38.2°C的FERT-TALP培养基中长时间培养（22小时）。我们的工作旨在开发一种方案，使用不同的培养基（FERT-TALP 和 INRA96）和培养温度（30 和 38.2°C），在 PHE 存在下培养 22 小时，以保持冷冻解冻马精子的质量参数。将四匹种公马的 12 个冷冻精子解冻，然后在 FERT-TALP 或 INRA96 中与 PHE 一起在 30 或 38.2°C 下培养 22 小时。结果表明，TM 明显更高（p

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.