Gene expression profiles and metabolic pathways responsible for male sterility in cybrid pummelo.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Rong Wang, Yang-Cao Shi, Bo Zhang, Wan-Rong Liu, Feng-Quan Tan, Fang Lu, Nan Jiang, Lai-Chao Cheng, Kai-Dong Xie, Xiao-Meng Wu, Wen-Wu Guo
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Abstract

Key message: Abnormal expression of genes regulating anther and pollen development and insufficient accumulation of male sterility (MS)- related metabolites lead to MS in cybrid pummelo Male sterility (MS) is a major cause of seedlessness in citrus, which is an important trait for fresh fruit. Understanding the mechanism of MS is important for breeding seedless citrus cultivars. In this study, we dissected the transcriptional, metabolic and physiological mechanisms of MS in somatic cybrid of pummelo (G1 + HBP). G1 + HBP exhibited severe male sterility, manifesting as retarded anther differentiation, abnormal anther wall development (especially tapetum and endothecium), and deficient pollen wall formation. In the anthers of G1 + HBP, the expression of genes regulating anther differentiation and tapetum development was abnormal, and the expression of genes regulating endothecium secondary lignification thickening and pollen wall formation was down-regulated. The transcription of genes involved in MS-related biological processes, such as jasmonic acid (JA) signaling pathway, primary metabolism, flavonoid metabolism, and programmed cell death, was altered in G1 + HBP anthers, and the accumulation of MS-associated metabolites, including fatty acids, amino acids, sugars, ATP, flavonols and reactive oxygen species (ROS), was down-regulated in G1 + HBP anthers. In summary, abnormal expression of key genes regulating anther and pollen development, altered transcription of key genes involved in MS-related metabolic pathways, and insufficient accumulation of MS-related metabolites together lead to MS in G1 + HBP. The critical genes and the metabolism pathways identified herein provide new insights into the formation mechanism of MS in citrus and candidate genes for breeding seedless citrus.

造成杂交柚雄性不育的基因表达谱和代谢途径
关键信息:调控花药和花粉发育的基因表达异常以及雄性不育(MS)相关代谢物积累不足导致杂交柚的雄性不育 雄性不育(MS)是柑橘无籽的主要原因,而无籽是新鲜水果的重要性状。了解 MS 的机理对于培育无籽柑橘栽培品种非常重要。本研究剖析了柚子体细胞杂交种(G1 + HBP)的转录、代谢和生理机制。G1 + HBP表现出严重的雄性不育,具体表现为花药分化迟缓、花药壁发育异常(尤其是叶柄和内皮)以及花粉壁形成不足。在 G1 + HBP 的花药中,调控花药分化和叶舌发育的基因表达异常,调控内皮层次生木质化增厚和花粉壁形成的基因表达下调。在 G1 + HBP 花药中,参与 MS 相关生物学过程(如茉莉酸(JA)信号通路、初级代谢、类黄酮代谢和细胞程序性死亡)的基因转录发生了改变,MS 相关代谢产物(包括脂肪酸、氨基酸、糖类、ATP、黄酮醇和活性氧(ROS))的积累在 G1 + HBP 花药中下调。总之,调控花药和花粉发育的关键基因表达异常、参与 MS 相关代谢途径的关键基因转录改变以及 MS 相关代谢物积累不足共同导致了 G1 + HBP 的 MS。本文鉴定的关键基因和代谢途径为了解柑橘 MS 的形成机制和培育无籽柑橘的候选基因提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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