Exogenous 5-aminolevulinic acid enhanced saline-alkali tolerance in pepper seedlings by regulating photosynthesis, oxidative damage, and glutathione metabolism.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Xueting Wang, Sizhen Yang, Baixue Li, Chunlin Chen, Jinling Li, Yichao Wang, Qingjie Du, Meng Li, Hu Wang, Juanqi Li, Jiqing Wang, HuaiJuan Xiao
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Abstract

Key message: A plant growth regulator, 5-aminolevulinic acid, enhanced the saline-alkali tolerance via photosynthetic, oxidative-reduction, and glutathione metabolism pathways in pepper seedlings. Saline-alkali stress is a prominent environmental problem, hindering growth and development of pepper. 5-Aminolevulinic acid (ALA) application effectively improves plant growth status under various abiotic stresses. Here, we evaluated morphological, physiological, and transcriptomic differences in saline-alkali-stressed pepper seedlings after ALA application to explore the impact of ALA on saline-alkali stress. The results indicated that saline-alkali stress inhibited plant growth, decreased biomass and photosynthesis, altered the osmolyte content and antioxidant system, and increased reactive oxygen species (ROS) accumulation and proline content in pepper seedlings. Conversely, the application of exogenous ALA alleviated this damage by increasing the photosynthetic rate, osmolyte content, antioxidant enzyme activity, and antioxidants, including superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase, and reducing glutathione to reduce ROS accumulation and malonaldehyde content. Moreover, the transcriptomic analysis revealed the differentially expressed genes were mainly associated with photosynthesis, oxidation-reduction process, and glutathione metabolism in saline-alkali stress + ALA treatment compared to saline-alkali treatment. Among them, the change in expression level in CaGST, CaGR, and CaGPX was close to the variation of corresponding enzyme activity. Collectively, our findings revealed the alleviating effect of ALA on saline-alkali stress in pepper seedlings, broadening the application of ALA and providing a feasible strategy for utilize saline-alkali soil.

外源5-氨基乙酰丙酸通过调节光合作用、氧化损伤和谷胱甘肽代谢增强辣椒幼苗的耐盐碱能力
关键信息植物生长调节剂5-氨基乙酰丙酸可通过光合作用、氧化还原和谷胱甘肽代谢途径增强辣椒幼苗的耐盐碱能力。盐碱胁迫是阻碍辣椒生长发育的一个突出环境问题。施用 5-氨基乙酰丙酸(ALA)能有效改善各种非生物胁迫下的植物生长状况。在此,我们评估了施用ALA后盐碱胁迫辣椒幼苗的形态、生理和转录组差异,以探讨ALA对盐碱胁迫的影响。结果表明,盐碱胁迫抑制了辣椒幼苗的生长,降低了生物量和光合作用,改变了渗透溶质含量和抗氧化系统,增加了活性氧(ROS)积累和脯氨酸含量。相反,施用外源 ALA 可通过提高光合速率、渗透压含量、抗氧化酶活性和抗氧化剂(包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶)以及还原谷胱甘肽来减少 ROS 积累和丙二醛含量,从而减轻这种损害。此外,转录组分析表明,与盐碱胁迫处理相比,盐碱胁迫+ALA处理的差异表达基因主要与光合作用、氧化还原过程和谷胱甘肽代谢有关。其中,CaGST、CaGR和CaGPX的表达水平变化与相应酶活性的变化接近。综上所述,我们的研究结果揭示了ALA对辣椒幼苗盐碱胁迫的缓解作用,拓宽了ALA的应用范围,为盐碱地的利用提供了可行的策略。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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