Unveiling the molecular symphony: MicroRNA160a-Auxin Response Factor 18 module orchestrates low potassium tolerance in banana (Musa acuminata L.)

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2024-10-11 DOI:10.1016/j.plantsci.2024.112288

Yi Tang , Hang Rong , Xingchen Jia , Yinglong Chen , Zishu Wang , Jinyi Wei , Chenyi Yang , Jianfu Liu , Mingyuan Wang , Hailing Yu , Qizhi Wang

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引用次数: 0

Abstract

Potassium (K) is an essential nutrient for the growth and development of most plants. In banana (Musa acuminata L.), microRNA160a (miR160a) is suggested to potentially contribute to the response to low K⁺ stress by modulating the auxin signaling pathway. However, further investigation is required to elucidate its specific regulatory mechanism. This study presents evidence highlighting the critical role of the miR160a-Auxin Response Factor 18 (ARF18) module in conferring low K⁺ tolerance in banana. Both miR160a and its predicted target gene ARF18 displayed elevated expression levels in banana roots, with their expression profiles significantly altered under low K⁺ stress. The inhibitory effect of mac-miR160a on the expression of MaARF18-like-2 was confirmed through tobacco transient transformation and dual-Luciferase reporter assay. Surprisingly, Arabidopsis lines overexpressing mac-miR160a (mac-miR160a OE) exhibited enhanced tolerance to low K⁺ stress. Conversely, Arabidopsis lines overexpressing MaARF18-like-2 (MaARF18-like-2 OE) displayed increased sensitivity to K⁺ deficiency. Additionally, RNA sequencing (RNA-seq) analysis revealed that MaARF18-like-2 mediates the response of Arabidopsis to low K⁺ stress by influencing the expression of genes associated with Ca²⁺, ion transport, and reactive oxygen species (ROS) signaling. In conclusion, our study provides novel insights into the molecular mechanism of the miR160a-ARF18-like-2 module in the plant response to low K⁺ stress.

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揭开分子交响乐的神秘面纱：MicroRNA160a-Auxin Response Factor 18 模块协调了香蕉（Musa acuminata L.）的低钾耐受性。

钾（K）是大多数植物生长和发育所必需的养分。在香蕉（Musa acuminata L.）中，microRNA160a（miR160a）被认为可能通过调节辅助素信号通路来促进对低 K+ 胁迫的响应。然而，要阐明其具体的调控机制还需要进一步的研究。本研究提出的证据强调了 miR160a-Auxin Response Factor 18（ARF18）模块在赋予香蕉低 K+耐受性中的关键作用。miR160a及其预测的靶基因ARF18在香蕉根中的表达水平都有所升高，在低K+胁迫下它们的表达谱发生了显著变化。通过烟草瞬时转化和双荧光素酶报告实验证实了mac-miR160a对MaARF18-like-2表达的抑制作用。令人惊讶的是，过表达 mac-miR160a 的拟南芥品系（mac-miR160a OE）对低 K+胁迫表现出更强的耐受性。相反，过表达 MaARF18-like-2 的拟南芥品系（MaARF18-like-2 OE）对 K+ 缺乏的敏感性增强。此外，RNA 测序（RNA-seq）分析表明，MaARF18-like-2 通过影响与 Ca2+、离子转运和活性氧（ROS）信号转导相关的基因表达，介导拟南芥对低 K+胁迫的响应。总之，我们的研究为miR160a-ARF18-like-2模块在植物对低K+胁迫响应中的分子机制提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.