Genome-wide analysis of Triticum aestivum bromodomain gene family and expression analysis under salt stress.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-15 DOI:10.1007/s00425-024-04549-1
Yueduo Wang, Shenghai Shen, Zhaoming Wu, Weiqi Tao, Wei Zhang, Pei Yu
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引用次数: 0

Abstract

Main conclusion: This study identified 82 wheat BRD genes, revealing both conserved evolutionary and functional characteristics across plant species and novel features specific to wheat. GTE8-12 cluster TaBRDs were found as positive response to salt stress. Bromodomain-containing proteins (BRDs) are crucial in histone acetylation "reading" and chromatin remodeling in eukaryotes. Despite some of their members showing importance in various biological processes in plants, our understanding of the BRD family in wheat (Triticum aestivum) remains limited. This study comprehensively analyzes the T. aestivum BRD (TaBRD) family. We identified 82 TaBRD genes in wheat genome encoding hydrophobic proteins with a conserved pocket structure. Phylogenetic analysis classified these genes into 16 distinct clusters, with conserved protein motifs and gene structures within clusters but diverse patterns across clusters. Gene duplication analysis revealed that whole-genome or segmental duplication events were the primary expansion mechanism for the TaBRD family, with purifying selection acting on these genes. Subcellular localization and Gene Ontology (GO) analyses indicated that TaBRD proteins are predominantly nuclear-localized and involved in transcription regulation and RNA metabolism. Promoter analysis and interaction network prediction suggested diverse regulatory mechanisms for TaBRDs. Notably, TaBRDs from the GTE8-12 cluster were enriched with cis-elements responsive to abscisic acid (ABA), methyl jasmonate (MeJA), and light, implying their involvement in physiological functions and abiotic stress responses. Expression analysis confirmed tissue-specific patterns and responsiveness to salinity stress. This comprehensive study enhances our understanding of the BRD family in higher plants and provides a foundation for developing salt-tolerant wheat varieties.

小麦溴链基因家族全基因组分析及盐胁迫下的表达分析
主要结论:这项研究发现了 82 个小麦 BRD 基因,揭示了植物物种间保守的进化和功能特征以及小麦特有的新特征。发现GTE8-12群TaBRDs对盐胁迫有积极的响应。含溴结构域蛋白(BRDs)在真核生物的组蛋白乙酰化 "阅读 "和染色质重塑过程中至关重要。尽管其中一些成员在植物的各种生物过程中显示出重要性,但我们对小麦(Triticum aestivum)中 BRD 家族的了解仍然有限。本研究全面分析了小麦 BRD(TaBRD)家族。我们在小麦基因组中发现了 82 个 TaBRD 基因,这些基因编码具有保守口袋结构的疏水蛋白。系统发育分析将这些基因分为 16 个不同的簇,簇内的蛋白基序和基因结构保持一致,但不同簇之间的模式各不相同。基因复制分析表明,全基因组或片段复制事件是TaBRD家族的主要扩展机制,纯化选择作用于这些基因。亚细胞定位和基因本体(GO)分析表明,TaBRD 蛋白主要定位在核内,参与转录调控和 RNA 代谢。启动子分析和相互作用网络预测表明,TaBRD 的调控机制多种多样。值得注意的是,GTE8-12集群中的TaBRDs富含对脱落酸(ABA)、茉莉酸甲酯(MeJA)和光有响应的顺式元件,这意味着它们参与了生理功能和非生物胁迫响应。表达分析证实了组织特异性模式和对盐度胁迫的响应。这项全面的研究加深了我们对高等植物中 BRD 家族的了解,为开发耐盐小麦品种奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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