MicroRNAs from Yishen Tongluo formula can repair sperm DNA damage caused by benzo(a)pyrene.

IF 3.9 3区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Pharmaceutical Biology Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI:10.1080/13880209.2024.2417002

Chenming Zhang, Ruimin Ma, Wenbang Liu, Sicheng Ma, Zulong Wang, Zixue Sun

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引用次数: 0

Abstract

Context: Plant microRNAs (miRNAs) present in Yishen Tongluo formula (YSTL, a traditional Chinese herbal medicine formula) are considered as potential therapeutic drugs for reducing the sperm DNA fragmentation index (DFI).

Objective: To study the effectiveness of plant miRNAs in YSTL for repairing mouse sperm DNA damage caused by benzo(a)pyrene (BaP).

Methods and materials: Twenty-four male SPF ICR (CD1) mice were divided into control, BaP and YSTL groups. A BaP-induced (100 mg/kg) sperm DNA damage model was established in the BaP and YSTL groups, and the mice in the YSTL group were treated with YSTL (23.78 g/kg) for 8 weeks. Sperm DFI was determined via a sperm chromatin structure assay (SCSA). MicroRNAs in the testes of the mice were analysed via RNA-seq, and the top four plant miRNAs were screened, identified and overexpressed in GC cells. The effects of plant miRNAs on the viability and DNA integrity of GC cells exposed to benzo(a)pyrene diol epoxide (BPDE) (1 μM) were tested using CCK8 and comet assays.

Results: Compared with that of the BaP group, the DFI of the YSTL group decreased (9.57% vs. 18.54%, F = 18.645, p = 0.0236). miR166-y, miR894-x, miR822-x and miR396-x were screened. The CCK8 and comet assays revealed that the DFI of the mimic group was significantly lower than that of the BPDE (IC₅₀ = 1.006 μM) group, with the most significant difference in the miR396-x group.

Discussion and conclusions: Plant miRNAs such as miR396-x can penetrate the blood-testis barrier through the digestive system to repair sperm DNA.

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益参通络方中的微RNA能修复苯并芘对精子DNA的损伤。

背景：宜神通络方（YSTL，一种传统中药配方）中的植物microRNAs（miRNAs）被认为是降低精子DNA碎片指数（DFI）的潜在治疗药物：方法与材料：24只雄性SPF ICR（SPF-ICR）：24只雄性SPF ICR（CD1）小鼠分为对照组、BaP组和YSTL组。在 BaP 组和 YSTL 组建立 BaP（100 毫克/千克）诱导的精子 DNA 损伤模型，YSTL 组小鼠接受 YSTL（23.78 克/千克）治疗 8 周。精子染色质结构测定法（SCSA）测定了精子的DFI。通过 RNA-seq 分析了小鼠睾丸中的微 RNA，筛选、鉴定并在 GC 细胞中过表达了前四种植物 miRNA。利用 CCK8 和彗星试验检测了植物 miRNA 对暴露于苯并（a）芘二元环氧化物（BPDE）（1 μM）的 GC 细胞的活力和 DNA 完整性的影响：与 BaP 组相比，YSTL 组的 DFI 有所下降（9.57% vs. 18.54%，F = 18.645，p = 0.0236）。CCK8 和彗星试验显示，模拟组的 DFI 明显低于 BPDE 组（IC50 = 1.006 μM），其中 miR396-x 组差异最显著：miR396-x等植物miRNA可通过消化系统穿透血睾屏障修复精子DNA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical Biology 医学-药学

CiteScore

6.70

自引率

2.60%

发文量

191

审稿时长

1 months

期刊介绍： Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.