Identification of circRNA CDR1as/miR-214-3p regulatory axis in Legg-Calvé-Perthes disease.

IF 3.4 2区医学 Q2 GENETICS & HEREDITY

Orphanet Journal of Rare Diseases Pub Date : 2024-10-15 DOI:10.1186/s13023-024-03394-5

Xia Lan, Ronghui Yu, Jianyun Xu

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引用次数: 0

Abstract

Background: Legg-Calvé-Perthes disease (LCPD) commonly occurs among adolescents, threatening their health. However, the potential mechanism underlying LCPD remains unclear. miR-214-3p is shown as a critical role in LCPD development with unspecified upstream regulators.

Methods: Levels of miR-214-3p and circCDR1as in healthy controls and LCPD patients were determined by qRT-PCR. The role of circCDR1as/miR-214-3p axis in LCPD was determined by testing the cell viability and apoptosis in TC28 cells and primary chondrocytes. Regulation between circCDR1as and miR-214-3p was examined by RIP and ChIP assays. The inflammatory response and angiogenesis were evaluated by M2 macrophage polarization and HUVECs tumor formation.

Results: circCDR1as was overexpressed in LCPD patients with a negative correlation with miR-214-3p. Inhibition of circCDR1as alleviated the cell viability and apoptosis of DEX-treated chondrocytes, stimulated M2 macrophage polarization and angiogenesis. miR-214-3p was proved as a downstream effector to participate in circCDR1as mediated actions. circCDR1as recruited PRC2 complex to epigenetically suppress miR-214-3p.

Conclusion: Our study illustrated the role and mechanism of circCDR1as in LCPD development by targeting miR-214-3p, highlighting its potential in the therapy for LCPD.

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鉴定 Legg-Calvé-Perthes 病中的 circRNA CDR1as/miR-214-3p 调节轴。

背景：莱格-卡尔维-珀特氏病（LCPD）通常发生在青少年中，威胁着他们的健康。miR-214-3p在LCPD的发展中起着关键作用，其上游调节因子尚未明确：方法：采用 qRT-PCR 方法测定健康对照组和 LCPD 患者体内 miR-214-3p 和 circCDR1as 的水平。通过检测 TC28 细胞和原代软骨细胞的细胞活力和凋亡，确定 circCDR1as/miR-214-3p 轴在 LCPD 中的作用。通过 RIP 和 ChIP 检测了 circCDR1as 和 miR-214-3p 之间的调控。结果：circCDR1as在LCPD患者中过表达，与miR-214-3p呈负相关。抑制circCDR1as可减轻DEX处理的软骨细胞的活力和凋亡，刺激M2巨噬细胞极化和血管生成：我们的研究说明了circCDR1as通过靶向miR-214-3p在LCPD发病中的作用和机制，凸显了其在LCPD治疗中的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Orphanet Journal of Rare Diseases 医学-医学：研究与实验

CiteScore

6.30

自引率

8.10%

发文量

418

审稿时长

4-8 weeks

期刊介绍： Orphanet Journal of Rare Diseases is an open access, peer-reviewed journal that encompasses all aspects of rare diseases and orphan drugs. The journal publishes high-quality reviews on specific rare diseases. In addition, the journal may consider articles on clinical trial outcome reports, either positive or negative, and articles on public health issues in the field of rare diseases and orphan drugs. The journal does not accept case reports.