Antimicrobial and antibiotic-potentiating effect of calcium peroxide nanoparticles on oral bacterial biofilms.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-10-15 DOI:10.1038/s41522-024-00569-7

Neha Bankar, Lorenz Latta, Brigitta Loretz, Bashar Reda, Johanna Dudek, Hendrik Hähl, Matthias Hannig, Claus-Michael Lehr

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Abstract

Bacterial biofilms represent a prominent biological barrier against physical and chemical attacks. Disturbing the anaerobic microenvironment within biofilms by co-delivery of oxygen appears as a promising strategy to enhance the activity of an antibiotic. Here, we report the effect of oxygen-producing calcium peroxide nanoparticles (CaO₂ NP) in combination with tobramycin sulfate (Tob). On Pseudomonas aeruginosa PAO1 biofilms in vitro, the additive effect of CaO₂ NP towards Tob activity enhanced biofilm eradication by 2 log compared to Tob alone. For natural biofilms grown in the oral cavity of human volunteers in situ, treatment by CaO₂ NP alone slightly increased the fraction of dead bacteria from 44% in various controls, including Tob alone, to 57%. However, the combination of CaO₂ NP with Tob further increased the fraction of dead bacteria to 69%. These data confirm the intrinsic antimicrobial and antibiotic-potentiating effect of CaO₂ NP also in a clinically relevant setting.

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过氧化钙纳米颗粒对口腔细菌生物膜的抗菌和抗生素增效作用。

细菌生物膜是抵御物理和化学攻击的重要生物屏障。通过联合输送氧气来扰乱生物膜内的厌氧微环境似乎是一种很有前景的增强抗生素活性的策略。在这里，我们报告了产生氧气的过氧化钙纳米颗粒（CaO2 NP）与硫酸妥布霉素（Tob）的结合效果。在体外铜绿假单胞菌 PAO1 生物膜上，与单独使用硫酸妥布霉素相比，CaO2 NP 对硫酸妥布霉素活性的叠加效应使生物膜的根除率提高了 2 log。对于在人体志愿者口腔中原位生长的天然生物膜，单独使用 CaO2 NP 处理可使死亡细菌的比例从各种对照组（包括单独使用 Tob）的 44% 略微提高到 57%。然而，将二氧化钙氮氧化物与 Tob 结合使用可进一步将细菌死亡比例提高到 69%。这些数据证实了二氧化钙氮氧化物在临床相关环境中的内在抗菌和抗生素增效作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.