Investigating the regulatory mechanism of glucose metabolism by ubiquitin-like protein MNSFβ.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Reports Pub Date : 2024-10-15 DOI:10.1007/s11033-024-10009-6

Megumi Kono, Kyoko Yamasaki, Morihiko Nakamura

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Abstract

Background: Monoclonal nonspecific suppressor factor β (MNSFβ), a ubiquitously expressed member of the ubiquitin-like protein family, is associated with diverse cell regulatory functions. It has been implicated in glycolysis regulation and cell proliferation enhancement in the macrophage-like cell line Raw264.7. This study aims to show that HIF-1α regulates MNSFβ-mediated metabolic reprogramming.

Methods and results: In Raw264.7 cells, MNSFβ siRNA increased the oxygen consumption rate and reactive oxygen species (ROS) production but decreased ATP levels. Cells with MNSFβ knockdown showed a markedly increased ATP reduction rate upon the addition of oligomycin, a mitochondrial ATP synthase inhibitor. In addition, MNSFβ siRNA decreased the expression levels of mRNA and protein of HIF-1α-a regulator of glucose metabolism. Evaluation of the effect of MNSFβ on glucose metabolism in murine peritoneal macrophages revealed no changes in lactate production, glucose consumption, or ROS production.

Conclusion: MNSFβ affects both glycolysis and mitochondrial metabolism, suggesting HIF-1α involvement in the MNSFβ-regulated glucose metabolism in Raw264.7 cells.

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研究泛素样蛋白MNSFβ对葡萄糖代谢的调控机制

背景：单克隆非特异性抑制因子β（MNSFβ）是泛素样蛋白家族中的一个泛素表达成员，与多种细胞调控功能有关。它与巨噬细胞样细胞系 Raw264.7 的糖酵解调节和细胞增殖增强有关。本研究旨在证明 HIF-1α 可调控 MNSFβ 介导的代谢重编程：在 Raw264.7 细胞中，MNSFβ siRNA 增加了氧消耗率和活性氧（ROS）的产生，但降低了 ATP 水平。在加入线粒体 ATP 合成酶抑制剂寡霉素后，MNSFβ siRNA 被敲除的细胞显示 ATP 减少率明显增加。此外，MNSFβ siRNA 还降低了葡萄糖代谢调节因子 HIF-1α 的 mRNA 和蛋白表达水平。通过评估 MNSFβ 对小鼠腹腔巨噬细胞葡萄糖代谢的影响，发现乳酸生成、葡萄糖消耗和 ROS 生成均无变化：结论：MNSFβ同时影响糖酵解和线粒体代谢，表明HIF-1α参与了MNSFβ调节Raw264.7细胞的糖代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.