P Zeebul Trinita Shannan, Susan G Suganya, M Ramesh, E Angel Jemima
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引用次数: 0
Abstract
Background: Most anticancer drugs possess the capacity to control precise molecular processes and gene-regulated protein expressions, which could enhance the efficacy of cancer treatments. Hirudinaria manillensis is prevalent in South Asia and has been employed for several decades in medical conditions based on its curative benefits.
Methods and results: The present study aimed to explore the molecular regulation of the target EGFR gene, and the protein expression of EGFR on the HeLa cell line. To achieve our aim, quantitative real-time PCR and immunocytochemistry assays were conducted. Interestingly, qRT-PCR results confirmed the downregulation of the EGFR gene on the HeLa cells in comparison with the β- actin internal control gene. Furthermore, immunocytochemistry assay results confirmed the decreasing level of EGFR gene expression in the HeLa cells.
Conclusion: Therefore, Hirudinaria manillensis could be a promising anticancer candidate for cervical cancer treatment.
新型 Hirudinaria manillensis 提取物对 HeLa 细胞中表皮生长因子受体基因及其蛋白表达的分子调控作用。
背景：大多数抗癌药物都具有精确控制分子过程和基因调控蛋白表达的能力，这可以提高癌症治疗的疗效。Hirudinaria manillensis盛产于南亚，几十年来一直被用于治疗各种疾病：本研究旨在探讨目标表皮生长因子受体基因的分子调控以及表皮生长因子受体蛋白在 HeLa 细胞系中的表达。为了达到这一目的，我们进行了实时定量 PCR 和免疫细胞化学分析。有趣的是，与β-肌动蛋白内对照基因相比，qRT-PCR 结果证实了表皮生长因子受体基因在 HeLa 细胞上的下调。此外，免疫细胞化学分析结果也证实了 HeLa 细胞中表皮生长因子受体基因表达水平的下降：因此，马氏葫芦巴可能是治疗宫颈癌的一种有前途的抗癌候选药物。
期刊介绍：
Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.
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