Combating wheat yellow mosaic virus through microbial interactions and hormone pathway modulations.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY
Fangyan Wang, Haoqing Zhang, Hongwei Liu, Chuanfa Wu, Yi Wan, Lifei Zhu, Jian Yang, Peng Cai, Jianping Chen, Tida Ge
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引用次数: 0

Abstract

Background: The rhizosphere microbiome is critical for promoting plant growth and mitigating soil-borne pathogens. However, its role in fighting soil-borne virus-induced diseases, such as wheat yellow mosaic virus (WYMV) transmitted by Polymyxa graminis zoospores, remains largely underexplored. In this study, we hypothesized that during viral infections, plant microbiomes engage in critical interactions with plants, with key microbes playing vital roles in maintaining plant health. Our research aimed to identify microbial taxa that not only suppress the disease but also boost wheat yield by using a blend of techniques, including field surveys, yield assessments, high-throughput sequencing of plant and soil microbiomes, microbial isolation, hydroponic experiments, and transcriptome sequencing.

Results: We found that, compared with roots and leaves, the rhizosphere microbiome showed a better performance in predicting wheat yield and the prevalence of P. graminis and WYMV across the three WYMV-impacted regions in China. Using machine learning, we found that healthy rhizospheres were marked with potentially beneficial microorganisms, such as Sphingomonas and Allorhizobium-Neorhizobium-Parararhizobium-Rhizobium, whereas diseased rhizospheres were associated with a higher prevalence of potential pathogens, such as Bipolaris and Fusicolla. Structural equation modeling showed that these biomarkers both directly and indirectly impacted wheat yield by modulating the rhizosphere microbiome and P. graminis abundance. Upon re-introduction of two key healthy rhizosphere biomarkers, Sphingomonas azotifigens and Rhizobium deserti, into the rhizosphere, wheat growth and health were enhanced. This was attributed to the up-regulation of auxin and cytokinin signaling pathways and the regulation of jasmonic acid and salicylic acid pathways during infections.

Conclusions: Overall, our study revealed the critical role of the rhizosphere microbiome in combating soil-borne viral diseases, with specific rhizosphere microbes playing key roles in this process. Video Abstract.

通过微生物相互作用和激素途径调节抗击小麦黄曲霉病毒。
背景:根圈微生物群对促进植物生长和减轻土传病原体至关重要。然而,它在抗击土传病毒引起的病害(如由禾谷多粘孢子菌传播的小麦黄曲霉病毒(WYMV))方面的作用在很大程度上仍未得到充分探索。在本研究中,我们假设在病毒感染期间,植物微生物群与植物发生了关键的相互作用,其中关键微生物在维持植物健康方面发挥了重要作用。我们的研究旨在通过田间调查、产量评估、植物和土壤微生物组的高通量测序、微生物分离、水培实验和转录组测序等多种技术,找出不仅能抑制病害,还能提高小麦产量的微生物类群:结果:我们发现,与根部和叶片相比,根圈微生物组在预测中国三个受 WYMV 影响地区的小麦产量以及禾谷粉虱和 WYMV 感染率方面表现更佳。通过机器学习,我们发现健康的根瘤菌群中含有潜在的有益微生物,如鞘氨单胞菌和全缘根瘤菌-根瘤菌-副根瘤菌-根瘤菌,而病害根瘤菌群中则含有较多的潜在病原体,如双极体和镰刀菌。结构方程模型显示,这些生物标志物通过调节根瘤微生物群和禾谷粉虱的丰度,直接和间接地影响了小麦的产量。在向根瘤菌层重新引入两种关键的健康根瘤菌生物标志物--叠氮单胞菌(Sphingomonas azotifigens)和沙漠根瘤菌(Rhizobium deserti)后,小麦的生长和健康得到了改善。这归因于感染过程中辅助素和细胞分裂素信号通路的上调以及茉莉酸和水杨酸通路的调节:总之,我们的研究揭示了根瘤微生物群在抗击土传病毒病中的关键作用,特定的根瘤微生物在这一过程中发挥了关键作用。视频摘要
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来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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