Trivalent outer membrane vesicles-based combination vaccine candidate induces protective immunity against Campylobacter and invasive non-typhoidal Salmonella in adult mice.

IF 5.5 3区 医学 Q1 IMMUNOLOGY
Soumalya Banerjee, Prolay Halder, Sanjib Das, Suhrid Maiti, Jeffrey H Withey, Jiro Mitobe, Goutam Chowdhury, Kei Kitahara, Shin-Ichi Miyoshi, Asish Kumar Mukhopadhyay, Shanta Dutta, Hemanta Koley
{"title":"Trivalent outer membrane vesicles-based combination vaccine candidate induces protective immunity against Campylobacter and invasive non-typhoidal Salmonella in adult mice.","authors":"Soumalya Banerjee, Prolay Halder, Sanjib Das, Suhrid Maiti, Jeffrey H Withey, Jiro Mitobe, Goutam Chowdhury, Kei Kitahara, Shin-Ichi Miyoshi, Asish Kumar Mukhopadhyay, Shanta Dutta, Hemanta Koley","doi":"10.1007/s00430-024-00805-z","DOIUrl":null,"url":null,"abstract":"<p><p>Campylobacter and invasive non-typhoidal Salmonella (iNTS) are among the most common causative agents of gastroenteritis worldwide. As of now, no single combination licensed vaccine is available for public health use against both iNTS and Campylobacter species. Outer-membrane vesicles (OMVs) are nanoscale proteoliposomes released from the surface of gram-negative bacteria during log phase and harbor a variety of immunogenic proteins. Based on epidemiology of infections, we formulated a novel trivalent outer membrane vesicles (TOMVs)-based vaccine candidate against Campylobacter jejuni (CJ), Salmonella Typhimurium (ST) and Salmonella Enteritidis (SE). Isolated OMVs from CJ, ST and SE were combined in equal ratios for formulation of TOMVs and 5 µg of the developed vaccine candidate was used for intraperitoneal immunization of adult BALB/c mice. Immunization with TOMVs significantly activated both the humoral and cellular arm of adaptive immune response. Robust bactericidal effect was elicited by TOMVs immunized adult mice sera. TOMVs immunization induced long-term protective efficacy against CJ, ST and SE infections in mice. The study illustrates the ability of TOMVs-based combination immunogen in eliciting broad-spectrum protective immunity against prevalent Campylobacter and iNTS pathogens. According to the findings, TOMVs can work as a potent combination-based acellular vaccine candidate for amelioration of Campylobacter and iNTS-mediated gastroenteritis.</p>","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":5.5000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical Microbiology and Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00430-024-00805-z","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Campylobacter and invasive non-typhoidal Salmonella (iNTS) are among the most common causative agents of gastroenteritis worldwide. As of now, no single combination licensed vaccine is available for public health use against both iNTS and Campylobacter species. Outer-membrane vesicles (OMVs) are nanoscale proteoliposomes released from the surface of gram-negative bacteria during log phase and harbor a variety of immunogenic proteins. Based on epidemiology of infections, we formulated a novel trivalent outer membrane vesicles (TOMVs)-based vaccine candidate against Campylobacter jejuni (CJ), Salmonella Typhimurium (ST) and Salmonella Enteritidis (SE). Isolated OMVs from CJ, ST and SE were combined in equal ratios for formulation of TOMVs and 5 µg of the developed vaccine candidate was used for intraperitoneal immunization of adult BALB/c mice. Immunization with TOMVs significantly activated both the humoral and cellular arm of adaptive immune response. Robust bactericidal effect was elicited by TOMVs immunized adult mice sera. TOMVs immunization induced long-term protective efficacy against CJ, ST and SE infections in mice. The study illustrates the ability of TOMVs-based combination immunogen in eliciting broad-spectrum protective immunity against prevalent Campylobacter and iNTS pathogens. According to the findings, TOMVs can work as a potent combination-based acellular vaccine candidate for amelioration of Campylobacter and iNTS-mediated gastroenteritis.

基于三价外膜囊泡的候选联合疫苗可诱导成年小鼠对弯曲杆菌和侵袭性非伤寒沙门氏菌产生保护性免疫。
弯曲菌和侵袭性非伤寒沙门氏菌 (iNTS) 是全球最常见的肠胃炎致病菌。到目前为止,还没有一种获得许可的联合疫苗可用于公共卫生领域,同时预防 iNTS 和弯曲杆菌。外膜囊泡 (OMV) 是革兰氏阴性细菌在对数期从表面释放的纳米级蛋白脂质体,内含多种免疫原蛋白。根据感染的流行病学,我们制定了一种基于三价外膜囊泡 (TOMVs) 的新型候选疫苗,用于预防空肠弯曲菌 (CJ)、鼠伤寒沙门氏菌 (ST) 和肠炎沙门氏菌 (SE)。将从空肠弯曲菌、伤寒沙门氏菌和肠炎沙门氏菌中分离出的 OMVs 以等比例混合配制成 TOMVs,并使用 5 µg 所开发的候选疫苗对成年 BALB/c 小鼠进行腹腔免疫。用 TOMVs 进行免疫可显著激活适应性免疫反应的体液免疫和细胞免疫。经 TOMVs 免疫的成年小鼠血清具有强大的杀菌作用。TOMVs免疫可诱导小鼠对CJ、ST和SE感染产生长期保护效力。该研究表明,基于 TOMVs 的组合免疫原能够针对流行的弯曲杆菌和 iNTS 病原体激发广谱保护性免疫。研究结果表明,TOMVs 可以作为一种有效的、基于组合的无细胞疫苗候选物,用于改善弯曲杆菌和 iNTS 介导的肠胃炎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
10.60
自引率
0.00%
发文量
29
审稿时长
1 months
期刊介绍: Medical Microbiology and Immunology (MMIM) publishes key findings on all aspects of the interrelationship between infectious agents and the immune system of their hosts. The journal´s main focus is original research work on intrinsic, innate or adaptive immune responses to viral, bacterial, fungal and parasitic (protozoan and helminthic) infections and on the virulence of the respective infectious pathogens. MMIM covers basic, translational as well as clinical research in infectious diseases and infectious disease immunology. Basic research using cell cultures, organoid, and animal models are welcome, provided that the models have a clinical correlate and address a relevant medical question. The journal also considers manuscripts on the epidemiology of infectious diseases, including the emergence and epidemic spreading of pathogens and the development of resistance to anti-infective therapies, and on novel vaccines and other innovative measurements of prevention. The following categories of manuscripts will not be considered for publication in MMIM: submissions of preliminary work, of merely descriptive data sets without investigation of mechanisms or of limited global interest, manuscripts on existing or novel anti-infective compounds, which focus on pharmaceutical or pharmacological aspects of the drugs, manuscripts on existing or modified vaccines, unless they report on experimental or clinical efficacy studies or provide new immunological information on their mode of action, manuscripts on the diagnostics of infectious diseases, unless they offer a novel concept to solve a pending diagnostic problem, case reports or case series, unless they are embedded in a study that focuses on the anti-infectious immune response and/or on the virulence of a pathogen.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信