Immunohistochemical expression of CYP11A1, CYP11B, CYP17, and HSD3B2 in functional and nonfunctional canine adrenocortical tumors

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2024-10-10 DOI:10.1111/jvim.17212

Frederik Allan, Alice H. Watson, Harriet M. Syme

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引用次数: 0

Abstract

Background

Functionality of human adrenal tumors is inferred by CYP11B1 (cortisol synthase) expression, CYP11B2 (aldosterone synthase) expression, or both.

Hypothesis/Objectives

Nonfunctional canine adrenal tumors have low expression of steroidogenic enzymes, whereas aldosterone-producing tumors express CYP11B, and cortisol-producing tumors express both CYP11B and CYP17.

Animals

Twenty-two client-owned dogs with adrenocortical tumors (ACT) (8 nonfunctional, 7-cortisol producing, 2 aldosterone-producing and 5 functional noncortisol producing) and 2 dogs with normal adrenal glands.

Methods

Retrospective case series. Adrenal functionality was determined from clinical signs and endocrine testing. CYP11A1, CYP11B, CYP17, and HSD3B2 expression was detected by immunohistochemistry on formalin-fixed paraffin-embedded adrenal tissue. Protein expression was semiquantified by 2 blinded observers using H-scoring (results reported as median [range]) and compared in nonfunctional and cortisol-producing adrenal tumors by Mann-Whitney U tests.

Results

CYP11A1, CYP11B, and HSD3B2 were present within all cortical layers of normal adrenal glands, and CYP17 was expressed within the zona fasciculata and zona reticularis. Expression of CYP11A1 (191.25 [97.5-270] vs. 175 [102.5-295] P = .69), CYP11B (190 [130-265] vs. 147.5 [95-202.5]; P = .07), CYP17 (177.5 [87.5-240] vs. 247.5 [55-292.5]; P = .40), and HSD3B2 (230 [47.5-295] vs. 277.5 [67.5-295]; P = .34) were not significantly different between cortisol-producing and nonfunctional ACT.

Conclusions and Clinical Importance

Our findings suggest it is not possible to determine functionality of canine ACT by immunohistochemistry for steroidogenic enzymes. Tumor size cannot be used to infer functionality of adrenal tumors.

Abstract Image

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功能性和非功能性犬肾上腺皮质肿瘤中 CYP11A1、CYP11B、CYP17 和 HSD3B2 的免疫组化表达。

背景：根据CYP11B1（皮质醇合成酶）、CYP11B2（醛固酮合成酶）或两者的表达推断人类肾上腺肿瘤的功能：无功能性犬肾上腺肿瘤的类固醇生成酶表达量较低，而醛固酮生成肿瘤表达 CYP11B，皮质醇生成肿瘤同时表达 CYP11B 和 CYP17：22只客户饲养的肾上腺皮质肿瘤（ACT）犬（8只无功能，7只分泌皮质醇，2只分泌醛固酮，5只分泌非皮质醇）和2只肾上腺正常的犬：方法：回顾性病例系列。根据临床症状和内分泌检测确定肾上腺功能。在福尔马林固定的石蜡包埋肾上腺组织上用免疫组化法检测 CYP11A1、CYP11B、CYP17 和 HSD3B2 的表达。由两名盲人观察员使用 H 评分对蛋白质表达进行半定量分析（结果以中位数[范围]报告），并通过 Mann-Whitney U 检验比较无功能性肾上腺肿瘤和皮质醇分泌性肾上腺肿瘤的表达情况：CYP11A1、CYP11B和HSD3B2存在于正常肾上腺的所有皮质层中，CYP17表达于筋膜区和网状区。CYP11A1（191.25 [97.5-270] vs. 175 [102.5-295] P = .69）、CYP11B（190 [130-265] vs. 147.5 [95-202.5]; P = .07）、CYP17（177.5 [87.5-240] vs. 247.5 [55-292.5]; P = .40）和 HSD3B2（230 [47.5-295] vs. 277.5 [67.5-295]；P = .34）在皮质醇分泌型和非功能型 ACT 之间无显著差异：我们的研究结果表明，无法通过类固醇生成酶的免疫组化来确定犬 ACT 的功能。肿瘤大小不能用来推断肾上腺肿瘤的功能性。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.