Activation of ionotropic and group I metabotropic glutamate receptors stimulates kisspeptin neuron activity in mice.

IF 3.3 4区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Robin J Bearss, Isabella A Oliver, Peighton N Neuman, Wahab I Abdulmajeed, Jennifer M Ackerman, Richard Piet
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Abstract

Different populations of hypothalamic kisspeptin (KISS1) neurons located in the rostral periventricular area of the third ventricle (RP3V) and arcuate nucleus (ARC) are thought to generate the sex-specific patterns of gonadotropin secretion. These neuronal populations integrate gonadal sex steroid feedback with internal and external cues relayed via the actions of neurotransmitters and neuropeptides. The excitatory amino acid neurotransmitter glutamate, the main excitatory neurotransmitter in the brain, plays a role in regulating gonadotropin secretion, at least partially through engaging KISS1 signaling. The expression and function of individual glutamate receptor subtypes in KISS1 neurons, however, are not well characterized. Here, we used GCaMP-based calcium imaging and patch-clamp electrophysiology to assess the impact of activating individual ionotropic (iGluR) and group I metabotropic (mGluR) glutamate receptors on KISS1 neuron activity in the mouse RP3V and ARC. Our results indicate that activation of all iGluR subtypes and of group I mGluRs, likely mGluR1, consistently drives activity in the majority of KISS1 neurons within the RP3V and ARC of males and females. Our results also revealed, somewhat unexpectedly, sex- and region-specific differences. Indeed, activating (S)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) type iGluRs evoked larger responses in female ARCKISS1 neurons than in their male counterparts whereas activating group I mGluRs induced larger responses in RP3VKISS1 neurons than in ARCKISS1 neurons in females. Together, our findings suggest that glutamatergic neurotransmission in KISS1 neurons, and its impact on the activity of these cells, might be sex- and region-dependent in mice.

激活离子型和 I 组代谢型谷氨酸受体可刺激小鼠吻肽神经元的活动。
位于第三脑室喙侧室周区(RP3V)和弓状核(ARC)的不同下丘脑吻肽(KISS1)神经元群被认为产生了促性腺激素分泌的性别特异性模式。这些神经元群通过神经递质和神经肽的作用,将性腺性激素反馈与内部和外部线索结合起来。兴奋性氨基酸神经递质谷氨酸是大脑中主要的兴奋性神经递质,在调节促性腺激素分泌方面发挥作用,至少部分是通过参与 KISS1 信号传导。然而,KISS1 神经元中各个谷氨酸受体亚型的表达和功能还没有得到很好的描述。在这里,我们利用基于 GCaMP 的钙成像和膜片钳电生理学评估了激活单个离子型(iGluR)和 I 组代谢型(mGluR)谷氨酸受体对小鼠 RP3V 和 ARC 中 KISS1 神经元活动的影响。我们的研究结果表明,所有 iGluR 亚型和 I 组 mGluR(可能是 mGluR1)的激活持续驱动着雌雄小鼠 RP3V 和 ARC 中大多数 KISS1 神经元的活动。我们的研究结果还意外地发现了性别和区域特异性差异。事实上,激活(S)-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸(AMPA)型 iGluRs 在雌性 ARCKISS1 神经元中诱发的反应比在雄性神经元中诱发的反应大,而激活 I 组 mGluRs 在雌性 RP3VKISS1 神经元中诱发的反应比在 ARCKISS1 神经元中诱发的反应大。我们的研究结果表明,小鼠 KISS1 神经元中的谷氨酸能神经递质及其对这些细胞活性的影响可能是性别和区域依赖性的。
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来源期刊
Journal of Neuroendocrinology
Journal of Neuroendocrinology 医学-内分泌学与代谢
CiteScore
6.40
自引率
6.20%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Journal of Neuroendocrinology provides the principal international focus for the newest ideas in classical neuroendocrinology and its expanding interface with the regulation of behavioural, cognitive, developmental, degenerative and metabolic processes. Through the rapid publication of original manuscripts and provocative review articles, it provides essential reading for basic scientists and clinicians researching in this rapidly expanding field. In determining content, the primary considerations are excellence, relevance and novelty. While Journal of Neuroendocrinology reflects the broad scientific and clinical interests of the BSN membership, the editorial team, led by Professor Julian Mercer, ensures that the journal’s ethos, authorship, content and purpose are those expected of a leading international publication.
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