Application of Fluorescent Composite Materials as a Sustained Release System in Treatment of Polycystic Ovary Syndrome.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2024-10-19 DOI:10.1007/s10895-024-03993-2

Lin Wang, Liangshan Mu, Yao Ye, Jun Xu, Xutong Zou

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引用次数: 0

Abstract

Polycystic ovary syndrome (PCOS) is a multifactorial disease characterized by oxidative stress and follicular dysfunction, leading to menstrual irregularities, hyperandrogenism, and infertility. Traditional drug delivery methods often result in drug loss and side effects on normal tissues. To address these issues, we synthesized two novel Co(II)-containing coordination polymers (CPs), {[Co(L)(H₂O)₂]·2H₂O}n (1) and {[Co(L)(H₂O)₂]·1.5H₂O}n (2), through the reaction of the T-shaped ligand (4 - 3'-pyridyl-,6 - 4'-carboxylphenyl)picolinic acid (H2L) with Co(NO₃)₂·6H₂O via a solvothermal process. Fluorescence spectroscopy revealed that the fluorescence emission of the CPs originates from the ligand, indicating their potential application as blue fluorescence materials. Subsequently, we encapsulated these CPs with hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) hydrogels to create two types of metal gel particles carrying spironolactone (HA/CMCS-CPs@spironolactone). SEM and TEM analyses showed that the material consists of tightly stacked sheet-like structures with an average size of approximately 100 nm. Thermogravimetric analysis (TGA) indicated that the material begins to decompose at around 115 °C, demonstrating good thermal stability. We assessed the inhibitory effects of these materials on oxidative stress induced by PCOS. The results showed that both types of spironolactone-loaded metal gel particles significantly reduced malondialdehyde (MDA) levels, particularly the particles constructed with CP2. HA/CMCS-CP1@spironolactone reduced MDA levels by approximately 17% and 46% at low and high concentrations, respectively, while HA/CMCS-CP2@spironolactone decreased MDA levels by about 55% and 39% at high and low concentrations, respectively. Therefore, the novel drug delivery system reported in this study has the potential to become a safe and effective option for the localized treatment of PCOS.

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荧光复合材料作为缓释系统在多囊卵巢综合征治疗中的应用

多囊卵巢综合征（PCOS）是一种以氧化应激和卵泡功能障碍为特征的多因素疾病，可导致月经不调、高雄激素症和不孕症。传统的给药方法往往会导致药物流失，并对正常组织产生副作用。为了解决这些问题，我们合成了两种新型含 Co(II) 配位聚合物 (CP)：{[Co(L)(H2O)2]-2H2O}n (1) 和 {[Co(L)(H2O)2]-1.5H2O}n (2)，它们是由 T 型配体（4-3'-吡啶基-,6-4'-羧基苯基）皮啉酸 (H2L) 与 Co(NO3)2-6H2O 通过溶解热反应生成的。荧光光谱显示，氯化石蜡的荧光发射源于配体，这表明氯化石蜡具有作为蓝色荧光材料的应用潜力。随后，我们用透明质酸（HA）和羧甲基壳聚糖（CMCS）水凝胶包裹这些氯化石蜡，制成了两种携带螺内酯的金属凝胶颗粒（HA/CMCS-CPs@spironolactone）。SEM 和 TEM 分析表明，该材料由紧密堆叠的片状结构组成，平均尺寸约为 100 nm。热重分析（TGA）表明，该材料在 115 °C 左右开始分解，显示出良好的热稳定性。我们评估了这些材料对 PCOS 诱导的氧化应激的抑制作用。结果表明，两种类型的螺内酯负载金属凝胶颗粒都能显著降低丙二醛（MDA）水平，尤其是用 CP2 构建的颗粒。HA/CMCS-CP1@spironolactone 在低浓度和高浓度下分别降低了约 17% 和 46% 的 MDA 水平，而 HA/CMCS-CP2@spironolactone 在高浓度和低浓度下分别降低了约 55% 和 39% 的 MDA 水平。因此，本研究中报道的新型给药系统有望成为局部治疗多囊卵巢综合症的一种安全有效的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.