Bacillus lipopeptides inhibit lipase activity and promote 3T3-L1 preadipocyte differentiation.

IF 5.6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Meichun Chen, Deju Chen, Rongfeng Xiao, Xuefang Zheng, Bo Liu, Jieping Wang
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引用次数: 0

Abstract

Bacillus lipopeptides have been reported to display anti-obesity effects. In the present study, Lipopeptides from Bacillus velezensis FJAT-45028 that consisted of iturin, fengycin and surfactin were reported. The lipopeptides exhibited a strong lipase inhibition activity in a concentration-dependent manner with a half maximal inhibitory concentration of 0.012 mg/mL, and the inhibition mechanism and type were reversible and competitive, respectively. Results of CCK8 assay showed that 3T3-L1 preadipocyte cells were completely viable under treatment of 0.050-0.2 mg/mL lipopeptides for 24 or 48 h. It was found that the lipopeptides could increase lipid droplets in the differentiated 3T3-L1 adipocytes in tested concentration and suppress the expression of peroxisome proliferator-activated receptor gamma (PPARγ). These results indicated the potential anti-obesity mechanism of the tested lipopeptides might be to inhibit lipase activity but not to suppress lipid accumulation in the adipocytes. Moreover, the lipopeptides could elevate glucose utilisation by 14.43%-33.81% in the differentiated 3T3-L1 adipocytes.

芽孢杆菌脂肽抑制脂肪酶活性并促进 3T3-L1 前脂肪细胞分化
据报道,芽孢杆菌脂肽具有抗肥胖作用。本研究报告了来自韦勒氏芽孢杆菌(Bacillus velezensis)FJAT-45028的脂肽,包括伊图灵(iturin)、芬吉霉素(fengycin)和表面活性素(surfactin)。这些脂肽具有很强的脂肪酶抑制活性,其半数最大抑制浓度为 0.012 mg/mL,抑制机制和类型分别为可逆性和竞争性。CCK8测定结果表明,在0.050-0.2 mg/mL脂肽处理24或48小时后,3T3-L1前脂肪细胞完全存活;在测试浓度下,脂肽可增加分化的3T3-L1脂肪细胞中的脂滴,并抑制过氧化物酶体增殖激活受体γ(PPARγ)的表达。这些结果表明,受试脂肽的潜在抗肥胖机制可能是抑制脂肪酶活性,而不是抑制脂肪细胞中的脂质积累。此外,这些脂肽还能使分化的 3T3-L1 脂肪细胞的葡萄糖利用率提高 14.43% 至 33.81%。
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来源期刊
CiteScore
10.30
自引率
10.70%
发文量
195
审稿时长
4-8 weeks
期刊介绍: Journal of Enzyme Inhibition and Medicinal Chemistry publishes open access research on enzyme inhibitors, inhibitory processes, and agonist/antagonist receptor interactions in the development of medicinal and anti-cancer agents. Journal of Enzyme Inhibition and Medicinal Chemistry aims to provide an international and interdisciplinary platform for the latest findings in enzyme inhibition research. The journal’s focus includes current developments in: Enzymology; Cell biology; Chemical biology; Microbiology; Physiology; Pharmacology leading to drug design; Molecular recognition processes; Distribution and metabolism of biologically active compounds.
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