Utility of Microwave-Synthesized Silver Nano Particles as Spectrofluorimetric Sensors for the Determination of Nano Concentrations of Favipravir: Application to Dosage Forms and Spiked Human Plasma.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Mona H Abo Zaid, Nahed El-Enany, Aziza E Mostafa, Ghada M Hadad, Fathalla Belal
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Abstract

A simple and facile microwave-assisted method was developed for the synthesis of highly fluorescent silver-nanoparticles (Ag-NPs). The synthesis of silver-nanoparticles depends on a redox reaction between silver nitrate and ascorbic acid using chitosan as a stabilizing agent. The produced Ag-NPs were characterized using Zeta potential and transmission electron microscope micrograph where they are spherical in shape with smooth surface morphology and size of 26.81 ± 2.2 nm. Favipiravir (FAV) was found to cause an obvious enhancement in the fluorescence of Ag-NPs; hence, they were used for its spectrofluorimetric estimation. The fluorescence intensity was measured at 430 nm after excitation at 360 nm. Under optimum conditions, a good linear relationship was accomplished between the FAV concentration and the fluorescence intensity in a range of (5.0-200.0) ng/mL with a limit of detection of 1.59 ng/mL. The method was successfully applied for the assay of the drug in its commercial tablets and spiked human plasma samples, and the results obtained were satisfactory.

微波合成的银纳米颗粒作为光谱荧光传感器测定法维拉韦纳米浓度的实用性:应用于剂型和加标人体血浆。
研究人员开发了一种简单易行的微波辅助方法,用于合成高荧光银纳米粒子(Ag-NPs)。银纳米粒子的合成取决于硝酸银和抗坏血酸之间的氧化还原反应,使用壳聚糖作为稳定剂。利用 Zeta 电位和透射电子显微镜显微照片对所制备的 Ag-NPs 进行了表征,发现它们呈球形,表面形态光滑,大小为 26.81 ± 2.2 nm。发现法维拉韦(FAV)会明显增强 Ag-NPs 的荧光,因此将其用于光谱荧光测定。在 360 纳米激发后,在 430 纳米处测量荧光强度。在最佳条件下,FAV 浓度与荧光强度在 (5.0-200.0) ng/mL 范围内呈良好的线性关系,检出限为 1.59 ng/mL。该方法成功地应用于该药物的商品片剂和加标人体血浆样品的检测,结果令人满意。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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