Reactive Fluorescent and Colorimetric Probe for Highly Selective and Sensitive Detection of Hg²⁺ in Real Water Samples.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2024-10-19 DOI:10.1007/s10895-024-03992-3

Jiarui Yang, Kaiqiang Zhang, Yong Zhao, Yanxi Song, Yihan Wu, Hongqi Li

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引用次数: 0

Abstract

Construction of efficient chemosensors for highly specific and sensitive detection of mercury ions remains a great challenge. In this work a highly selective and sensitive probe CY was designed and synthesized by using coumarin fluorophore as the matrix and thioacetal moiety as the reactive recognition site for Hg²⁺. By virtue of the thiophilicity of Hg²⁺, probe CL could be hydrolyzed to deprotect and the thioacetal was transformed to the acyl group after the addition of Hg²⁺, the blue-green fluorescence was quenched and meanwhile the solution changed from light green to yellow. The detection limit of probe CY for Hg²⁺ was as low as 6.8 nM, and it could completely react with Hg²⁺ within 3 min. Moreover, probe CY exhibited good resistance against interference from competitive metal ions and biothiols, high stability in pH 1-11 and applicability for fluorogenic and chromogenic dual-modal detection of Hg²⁺ in real water samples over a broad range of pH 5-10.

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用于高选择性和高灵敏度检测真实水样中 Hg2+ 的反应性荧光和比色探针。

构建高效的化学传感器来高度特异和灵敏地检测汞离子仍然是一项巨大的挑战。本研究以香豆素荧光团为基质，以硫代乙缩醛为 Hg2+ 的反应识别位点，设计并合成了一种高选择性、高灵敏度的探针 CY。由于 Hg2+ 的亲硫性，探针 CL 在加入 Hg2+ 后可水解脱保护，硫代缩醛转化为酰基，蓝绿色荧光被淬灭，同时溶液由浅绿色变为黄色。探针 CY 对 Hg2+ 的检测限低至 6.8 nM，并能在 3 分钟内与 Hg2+ 完全反应。此外，探针 CY 对竞争性金属离子和生物硫醇具有良好的抗干扰性，在 pH 值为 1-11 的条件下具有很高的稳定性，可在 pH 值为 5-10 的大范围内用于真实水样中 Hg2+ 的荧光和显色双模式检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.