Serum Lipid Profile and Electrolytes Reference Intervals for Apparently Healthy Children and Adolescents in Addis Ababa, Ethiopia

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2024-10-21 DOI:10.1002/jcla.25116

Ousman Mohammed, Melkitu Kassaw, Endalkachew Befekadu, Letebrhan G/Egzeabher, Yosef Tolcha, Feyissa Challa, Adisu Kebede, Genet Ashebir, Mehari Meles, Fatuma Hassen, Biruk Zerfu, Dessie Abera, Abiy Belay, Fikirte Aboneh, Daniel Hailu, Workabeba Abebe, Kassu Desta, Mistire Wolde, Aster Tsegaye

{"title":"Serum Lipid Profile and Electrolytes Reference Intervals for Apparently Healthy Children and Adolescents in Addis Ababa, Ethiopia","authors":"Ousman Mohammed, Melkitu Kassaw, Endalkachew Befekadu, Letebrhan G/Egzeabher, Yosef Tolcha, Feyissa Challa, Adisu Kebede, Genet Ashebir, Mehari Meles, Fatuma Hassen, Biruk Zerfu, Dessie Abera, Abiy Belay, Fikirte Aboneh, Daniel Hailu, Workabeba Abebe, Kassu Desta, Mistire Wolde, Aster Tsegaye","doi":"10.1002/jcla.25116","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Accurate reference intervals generated from an apparently healthy population and stratified by crucial variables such as age and gender are required to guarantee appropriate interpretation of test results. Since there were no local reference intervals in the study area, the present study aimed to establish reference intervals on serum lipid profiles and electrolytes for children and adolescents in Addis Ababa.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This community-based cross-sectional study was conducted from April to October 2019. Laboratory analysis was performed using the automatic biochemical analyzer Cobas 6000 (c501) from Roche. According to Clinical and Laboratory Standards Institute (CLSI) guidelines, reference intervals for lipid profile and electrolyte tests for apparently healthy children and adolescents were established. We used a non-parametric method to calculate the 2.5th and 97.5th percentiles with a 90% confidence interval.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In children, the reference intervals for serum potassium, sodium, chloride, calcium, magnesium, and phosphate in mmol/L were 4.37–5.20, 137–145.50, 101.90–107.90, 2.34–2.70, 0.74–0.97, and 1.42–1.85, respectively; and for total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, the respective values were 100.76–171.70, 44.16–126.36, 60.60–105.60, and 31.60–53.70 in mg/dL, for both genders. For adolescents, the reference intervals were 4.03–5.58, 137–146, 98.90–120.90, 2.39–2.70, 0.73–0.96, and 0.96–1.80 for serum potassium, sodium, chloride, calcium, magnesium, and phosphate in mmol/L, respectively; and 97.20–189.10, 40.50–143.60, 41.70–120.90, and 21.30–57.0 in mg/dL for total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, respectively, for both genders.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The established reference intervals in the current study revealed that both the lower and upper limits contradicted the manufacturer values as well as the available literature. The study also discovered significant gender differences in reference values for TC, TG, LDL-C, potassium, phosphate, and chloride in the adolescent age groups.</p>\n </section>\n </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"38 22","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.25116","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Laboratory Analysis","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jcla.25116","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Accurate reference intervals generated from an apparently healthy population and stratified by crucial variables such as age and gender are required to guarantee appropriate interpretation of test results. Since there were no local reference intervals in the study area, the present study aimed to establish reference intervals on serum lipid profiles and electrolytes for children and adolescents in Addis Ababa.

Methods

This community-based cross-sectional study was conducted from April to October 2019. Laboratory analysis was performed using the automatic biochemical analyzer Cobas 6000 (c501) from Roche. According to Clinical and Laboratory Standards Institute (CLSI) guidelines, reference intervals for lipid profile and electrolyte tests for apparently healthy children and adolescents were established. We used a non-parametric method to calculate the 2.5th and 97.5th percentiles with a 90% confidence interval.

Results

In children, the reference intervals for serum potassium, sodium, chloride, calcium, magnesium, and phosphate in mmol/L were 4.37–5.20, 137–145.50, 101.90–107.90, 2.34–2.70, 0.74–0.97, and 1.42–1.85, respectively; and for total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, the respective values were 100.76–171.70, 44.16–126.36, 60.60–105.60, and 31.60–53.70 in mg/dL, for both genders. For adolescents, the reference intervals were 4.03–5.58, 137–146, 98.90–120.90, 2.39–2.70, 0.73–0.96, and 0.96–1.80 for serum potassium, sodium, chloride, calcium, magnesium, and phosphate in mmol/L, respectively; and 97.20–189.10, 40.50–143.60, 41.70–120.90, and 21.30–57.0 in mg/dL for total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, respectively, for both genders.

Conclusion

The established reference intervals in the current study revealed that both the lower and upper limits contradicted the manufacturer values as well as the available literature. The study also discovered significant gender differences in reference values for TC, TG, LDL-C, potassium, phosphate, and chloride in the adolescent age groups.

Abstract Image

查看原文本刊更多论文

埃塞俄比亚亚的斯亚贝巴健康儿童和青少年的血清血脂和电解质参考区间。

背景：为了保证对检测结果做出正确的解释，需要从表面健康的人群中得出准确的参考区间，并根据年龄和性别等关键变量进行分层。由于研究地区没有当地的参考区间，本研究旨在为亚的斯亚贝巴的儿童和青少年建立血清脂质和电解质的参考区间：这项基于社区的横断面研究于 2019 年 4 月至 10 月进行。使用罗氏公司生产的自动生化分析仪 Cobas 6000（c501）进行实验室分析。根据临床和实验室标准协会（CLSI）指南，为表面健康的儿童和青少年确定了血脂和电解质检测的参考区间。我们采用非参数法计算了 2.5 百分位数和 97.5 百分位数，置信区间为 90%：结果：在儿童中，血清钾、钠、氯、钙、镁和磷酸盐的参考区间（单位：mmol/L）分别为 4.37-5.20、137-145.50、101.90-107.90、2.34-2.70、0.74-0.97 和 1.42-1.85；总胆固醇的参考区间（单位：mmol/L）为 1.42-1.85。以毫克/分升为单位，男女总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白的参考值分别为 100.76-171.70、44.16-126.36、60.60-105.60 和 31.60-53.70。对于青少年，血清钾、钠、氯、钙、镁和磷酸盐的参考区间分别为 4.03-5.58、137-146、98.90-120.90、2.39-2.70、0.73-0.96 和 0.96-1.80（单位：毫摩尔/升）；以及 97.总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白分别为 97.20-189.10、40.50-143.60、41.70-120.90 和 21.30-57.0（毫克/分升）：本次研究中确定的参考区间显示，其下限和上限均与生产商提供的数值以及现有文献相矛盾。研究还发现，在青少年年龄组中，TC、TG、LDL-C、钾、磷酸盐和氯化物的参考值存在明显的性别差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.