Hoxc10-mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting

IF 5.3
Haoyue Song, Yujia Hao, Qingpeng Xie, Xiaohang Chen, Na Li, Jia Wang, Xiaoxuan Zhang, Yuan Zhang, Jinjia Hong, Shuyun Xue, Pengfei Zhang, Si Xie, Xing Wang
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引用次数: 0

Abstract

The Hox gene plays a crucial role in the bone development, determining their structure and morphology. Limb bone grafts expressing Hox positive genes are commonly used for free transplantation to repair Hox negative mandibular critical bone defects. However, the specific role of original Hox genes in newly formed bone during the cross-layer bone grafting healing process remains unexplored. Our findings demonstrate that femurs ectopically grafted into the mandibular environment retained a significant ability to differentiate into cartilage and form cartilaginous callus, which may be a key factor contributing to differences in bone graft healing. Hoxc10, an embryonic layer-specific genes, regulates cartilage formation during bone healing. Mechanistically, we observed Hoxc10 retention in co-cultured femoral BMSCs. Knocking out Hoxc10 narrows the bone gap and reduces cartilage formation. In summary, we reveal Hoxc10's ‘positional memory’ after adult cross-layer bone graft, influencing the outcomes of autologous bone graft.

Abstract Image

Hoxc10介导的 "位置记忆 "调节股骨异位移植后的软骨形成。
Hox 基因在骨骼发育中起着至关重要的作用,决定着骨骼的结构和形态。表达 Hox 阳性基因的肢骨移植物通常用于游离移植,以修复 Hox 阴性的下颌骨关键骨缺损。然而,在跨层骨移植愈合过程中,原始 Hox 基因在新形成骨中的具体作用仍有待探索。我们的研究结果表明,异位移植到下颌骨环境中的股骨保留了分化成软骨和形成软骨胼胝体的重要能力,这可能是导致骨移植愈合差异的关键因素。Hoxc10是胚胎层特异性基因,在骨愈合过程中调节软骨的形成。从机制上讲,我们观察到 Hoxc10 保留在共培养的股骨 BMSCs 中。敲除 Hoxc10 会缩小骨间隙并减少软骨的形成。总之,我们揭示了Hoxc10在成人跨层骨移植后的 "位置记忆",它影响着自体骨移植的结果。
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来源期刊
CiteScore
11.50
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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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