Resistance to carbapenems in the urban soil isolate Cupriavidus taiwanensis S2-1-W is associated with OXA-1206, a newly discovered carbapenemase.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2024-10-03 DOI:10.1093/jambio/lxae265

Nicolas V Lopez, Cristian Ruiz

{"title":"Resistance to carbapenems in the urban soil isolate Cupriavidus taiwanensis S2-1-W is associated with OXA-1206, a newly discovered carbapenemase.","authors":"Nicolas V Lopez, Cristian Ruiz","doi":"10.1093/jambio/lxae265","DOIUrl":null,"url":null,"abstract":"Aims: Cupriavidus isolates are found in environmental and clinical samples and are often resistant to carbapenems, which are last-resort antibiotics. However, their carbapenem-resistance molecular mechanisms remain unknown. This study aimed to (i) characterize and sequence the carbapenem-resistant soil isolate Cupriavidus taiwanensis S2-1-W to uncover its antibiotic resistance determinants; and (ii) clone and characterize a putative novel carbapenemase gene identified in this isolate.Methods and results: Antibiotic susceptibility testing of C. taiwanensis S2-1-W revealed that it was resistant to most carbapenems, other β-lactams, and aminoglycosides tested. Genome sequencing of this isolate revealed a complex chromosomal resistome that included multidrug efflux pump genes, one aminoglycoside transferase gene, and three β-lactamase genes. Among them, we identified a novel putative class D β-lactamase gene (blaOXA-1206) that is highly conserved among other sequenced C. taiwanensis isolates. Cloning and characterization of blaOXA-1206 confirmed that it encodes for a newly discovered carbapenemase (OXA-1206) that confers resistance to carbapenems and other β-lactams.Conclusion: Carbapenem-resistance in C. taiwanensis S2-1-W is associated with a newly discovered carbapenemase, OXA-1206.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxae265","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aims: Cupriavidus isolates are found in environmental and clinical samples and are often resistant to carbapenems, which are last-resort antibiotics. However, their carbapenem-resistance molecular mechanisms remain unknown. This study aimed to (i) characterize and sequence the carbapenem-resistant soil isolate Cupriavidus taiwanensis S2-1-W to uncover its antibiotic resistance determinants; and (ii) clone and characterize a putative novel carbapenemase gene identified in this isolate.

Methods and results: Antibiotic susceptibility testing of C. taiwanensis S2-1-W revealed that it was resistant to most carbapenems, other β-lactams, and aminoglycosides tested. Genome sequencing of this isolate revealed a complex chromosomal resistome that included multidrug efflux pump genes, one aminoglycoside transferase gene, and three β-lactamase genes. Among them, we identified a novel putative class D β-lactamase gene (blaOXA-1206) that is highly conserved among other sequenced C. taiwanensis isolates. Cloning and characterization of blaOXA-1206 confirmed that it encodes for a newly discovered carbapenemase (OXA-1206) that confers resistance to carbapenems and other β-lactams.

Conclusion: Carbapenem-resistance in C. taiwanensis S2-1-W is associated with a newly discovered carbapenemase, OXA-1206.

查看原文本刊更多论文

城市土壤中分离出的台湾铜绿微囊藻 S2-1-W 对碳青霉烯类的抗药性与新发现的碳青霉烯酶 OXA-1206 有关。

目的：环境和临床样本中都发现了铜绿微囊藻分离菌，它们通常对碳青霉烯类抗生素耐药，而碳青霉烯类抗生素是最后的抗生素。然而，它们对碳青霉烯类抗生素产生耐药性的分子机制仍然未知。本研究旨在1）对耐碳青霉烯类抗生素的土壤分离株台湾铜绿菌（Cupriavidus taiwanensis S2-1-W）进行特征描述和测序，以揭示其抗生素耐药性决定因素；以及 2）克隆并描述在该分离株中发现的一个假定的新型碳青霉烯酶基因：方法：对台湾金黄色葡萄球菌 S2-1-W 进行的抗生素敏感性测试表明，它对大多数碳青霉烯类、其他 β-内酰胺类和氨基糖苷类药物都具有耐药性。对该分离株的基因组测序发现了一个复杂的染色体抗药性基因组，其中包括多药外排泵基因、一个氨基糖苷转移酶基因和三个β-内酰胺酶基因。其中，我们发现了一个新的推定 D 类 β-内酰胺酶基因（blaOXA-1206），该基因在其他已测序的台湾金黄色葡萄球菌分离株中高度保守。blaOXA-1206 的克隆和特征描述证实，它编码一种新发现的碳青霉烯酶（OXA-1206），可产生对碳青霉烯类和其他 β-内酰胺类药物的耐药性：结论：台湾金线鲃 S2-1-W 的碳青霉烯耐药性与新发现的碳青霉烯酶 OXA-1206 有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.