Biofilms and microbiome profiles in chronic wounds: links to antibiotic use and wound severity in a Sri Lankan cohort.

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ayomi Dilhari, Paul M Campbell, Anuradha Munasinghe, Hannah Brown, Thilini D J Kaluarachchi, Chinthika Gunasekara, Sujatha Pathirage, Neluka Fernando, Deepaka Weerasekara, Gavin J Humphreys, Andrew J McBain, Manjula Weerasekera
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引用次数: 0

Abstract

Aims: We have characterized the microbiome of infected chronic diabetic wounds (CDWs), exploring associations with antibiotic use and wound severity in a Sri Lankan cohort.

Methods and results: Fifty CDW patients were enrolled, 38 of whom received antibiotics. Tissue biopsies were analysed by microbiome profiling, and wounds were graded using the University of Texas Wound Grading System. Biofilm presence was assessed in 20 wounds. The microbiome was largely dominated by Enterobacteriaceae, Pseudomonadaceae, Streptococcaceae, and Corynebacteriaceae. Proteobacteria levels were significantly higher in antibiotic-treated wounds (P = .019), with increased Pseudomonas abundance. Wounds were categorized as grade 1 (10), grade 2 (29), and grade 3 (11). Alpha diversity varied by wound grade (P = .015), with grade 2 wounds showing the highest diversity and grade 3 the lowest. All 20 tested wounds were biofilm-positive, and community composition varied more in antibiotic-treated wounds (P = .004).

Conclusions: CDW microbiomes were dominated by Enterobacteriaceae and Pseudomonadaceae, with elevated Proteobacteria in antibiotic-treated wounds. Alpha diversity correlated with wound severity, peaking in grade 2 wounds. The high prevalence of biofilms in wounds underscores the need for management of CDWs that address microbial complexity.

慢性伤口中的生物膜和微生物组概况:斯里兰卡队列中抗生素使用和伤口严重程度的关联。
目的:我们对受感染的慢性糖尿病伤口(CDW)的微生物组进行了特征描述,并在斯里兰卡队列中探讨了与抗生素使用和伤口严重程度的关系:方法:共招募了 50 名 CDW 患者,其中 38 人接受了抗生素治疗。通过微生物组分析对组织活检进行了分析,并使用德克萨斯大学伤口分级系统对伤口进行了分级。对 20 处伤口的生物膜存在情况进行了评估。微生物组主要以肠杆菌科、假单胞菌科、链球菌科和棒状杆菌科为主。经抗生素治疗的伤口中,蛋白杆菌含量明显较高(p = 0.019),假单胞菌含量也有所增加。伤口分为 1 级(10 个)、2 级(29 个)和 3 级(11 个)。α多样性因伤口等级而异(p = 0.015),2级伤口的多样性最高,3级最低。所有 20 个受测伤口的生物膜均呈阳性,抗生素治疗伤口的群落组成差异更大(p = 0.004):结论:CDW 微生物群落以肠杆菌科和假单胞菌科为主,抗生素治疗的伤口中变形菌增多。α多样性与伤口严重程度相关,在2级伤口中达到高峰。伤口中生物膜的高流行率凸显了针对微生物复杂性进行 CDWs 管理的必要性。
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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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