Emerging Echinococcus tapeworms: fecal PCR detection of Echinococcus multilocularis in 26 dogs from the United States and Canada (2022-2024).

IF 1.6 2区 农林科学 Q2 VETERINARY SCIENCES
Michelle D Evason, Andrew S Peregrine, Emily J Jenkins, Cecilia E Lozoya, Lisa Langs Rund, J Scott Weese, Pablo D Jimenez Castro, Christian M Leutenegger
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引用次数: 0

Abstract

Objective: To report quantitative PCR (qPCR) detection of Echinococcus multilocularis DNA in fecal samples from 26 dogs in the US and Canada.

Animals: 26 dogs with fecal samples submitted for parasite screening by qPCR.

Clinical presentation: Dog signalment, presenting concern, preventive care, and outcomes were obtained from the primary veterinarian via email or telephone, where available.

Results: Echinococcus multilocularis was detected in 26 of 2,333,797 dog fecal samples by reference laboratory fecal qPCR surveillance between March 2022 and July 2024. Seventeen E multilocularis-detected samples were sequenced as European haplotypes (E3/E4). Taenia-type eggs were identified by zinc sulfate centrifugal flotation in 8 of 17 samples (47%). Dogs were from the US (n = 16) and Canada (10). Ten dogs had gastrointestinal signs (diarrhea) reported on initial presentation. Clinical history revealed that some dogs were receiving a monthly antiparasitic preventive in the 6-month period prior to sampling (n = 10) and had regular wildlife (rodent) exposure (13). Twenty-five dogs were subsequently confirmed to have received treatment with praziquantel for detected E multilocularis, and 25 of these dogs were qPCR negative 3 to 5 weeks after treatment.

Clinical relevance: Veterinary awareness of endemic risk regions for E multilocularis and its emergence in novel areas (Colorado, Nevada, Wyoming, Montana, Illinois, Washington, Idaho, Kansas, and Oregon) are key for One Health. Dogs can serve as sentinels for Echinococcus tapeworm risk, and detection of E multilocularis tapeworms in dogs through routine qPCR fecal screening can alert clinicians to zoonotic concern and common environmental exposure risk.

新出现的棘球绦虫:在美国和加拿大的 26 只狗(2022-2024 年)中通过粪便 PCR 检测到多角棘球绦虫。
目标:报告美国和加拿大 26 只狗粪便样本中多棘球蚴 DNA 的定量 PCR (qPCR) 检测结果。动物:26 只狗的粪便样本通过 qPCR 进行寄生虫筛查:临床表现:通过电子邮件或电话(如有)从主治兽医处获得狗的信号、症状、预防性护理和结果:结果:2022 年 3 月至 2024 年 7 月期间,通过参考实验室粪便 qPCR 监测,在 2,333,797 份犬粪便样本中的 26 份中检测出多棘球蚴。检测到的 17 份多角孢子虫样本被测序为欧洲单倍型 (E3/E4)。17 份样本中有 8 份(47%)通过硫酸锌离心浮选法鉴定出了嗒嗒虫卵。这些狗来自美国(16 只)和加拿大(10 只)。十只狗在初次发病时出现了胃肠道症状(腹泻)。临床病史显示,一些狗在采样前的 6 个月内每月接受一次抗寄生虫预防治疗(10 只),并经常接触野生动物(啮齿动物)(13 只)。25只狗随后被证实接受过吡喹酮治疗,以治疗检测出的多角体圆线虫,其中25只狗在治疗后3至5周qPCR呈阴性:临床意义:兽医对多角体圆线虫流行风险地区的认识以及新地区(科罗拉多州、内华达州、怀俄明州、蒙大拿州、伊利诺伊州、华盛顿州、爱达荷州、堪萨斯州和俄勒冈州)出现多角体圆线虫的情况对 "一体健康 "至关重要。狗可以作为棘球绦虫风险的哨兵,通过常规 qPCR 粪便筛查检测狗体内的多角体棘球绦虫可以提醒临床医生注意人畜共患病问题和常见的环境暴露风险。
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来源期刊
CiteScore
1.60
自引率
15.80%
发文量
539
审稿时长
6-16 weeks
期刊介绍: Published twice monthly, this peer-reviewed, general scientific journal provides reports of clinical research, feature articles and regular columns of interest to veterinarians in private and public practice. The News and Classified Ad sections are posted online 10 days to two weeks before they are delivered in print.
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