Paenibacillus suaedae sp. nov. and Paenibacillus violae sp. nov., isolated from the roots of Suaeda japonica Makino and Viola mandshurica W. Becker with plant growth-promoting potential.
Inhyup Kim, Sunho Park, Yonghee Jung, Haejin Woo, Hyunji Lee, Subin Yook, Taegun Seo
{"title":"<i>Paenibacillus suaedae</i> sp. nov. and <i>Paenibacillus violae</i> sp. nov., isolated from the roots of <i>Suaeda japonica</i> Makino and <i>Viola mandshurica</i> W. Becker with plant growth-promoting potential.","authors":"Inhyup Kim, Sunho Park, Yonghee Jung, Haejin Woo, Hyunji Lee, Subin Yook, Taegun Seo","doi":"10.1099/ijsem.0.006542","DOIUrl":null,"url":null,"abstract":"<p><p>Two novel Gram-positive strains, chi10<sup>T</sup> and PFR10<sup>T</sup>, were isolated from the roots of <i>Suaeda japonica</i> Makino and <i>Viola mandshurica</i> W. Becker, respectively. The strains are facultatively aerobic, rod-shaped, motile via peritrichous flagella and endospore-forming. Strains chi10<sup>T</sup> and PFR10<sup>T</sup> showed the highest 16S rRNA gene sequence similarity to <i>Paenibacillus alvei</i> DSM 29<sup>T</sup> (99.0%) and <i>Paenibacillus planticolens</i> LMG 31457<sup>T</sup> (99.3%). Furthermore, the phylogenetic and phylogenomic analyses demonstrated that strains chi10<sup>T</sup> and PFR10<sup>T</sup> were closely related to the genus <i>Paenibacillus</i>. The digital DNA-DNA hybridization (dDDH) values for strains used in the phylogenetic analysis and strain chi10<sup>T</sup> ranged from 19.6 to 28.4%, while for strain PFR10<sup>T</sup>, the values ranged from 19.8 to 53.5%, according to the Genome-to-Genome Distance Calculator 3.0, which were all less than 70%. Additionally, the dDDH value between strains chi10<sup>T</sup> and PFR10<sup>T</sup> was 22.0%, confirming that they are different species of the genus <i>Paenibacillus</i>. The average nucleotide identity between chi10<sup>T</sup> and PFR10<sup>T</sup> and other species of the genus <i>Paenibacillus</i> were 68.4-84.7% and 67.8-93.6%, respectively. In addition, the major polar lipids of chi10<sup>T</sup> and PFR10<sup>T</sup> consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acid profile of strain chi10<sup>T</sup> was iso-C<sub>15 : 0</sub> and anteiso-C<sub>15 : 0</sub>, whereas the major fatty acid profile of strain PFR10<sup>T</sup> was iso-C<sub>15 : 0</sub>, anteiso-C<sub>15 : 0</sub> and iso-C<sub>16 : 0</sub>. The sole respiratory quinone in strains chi10<sup>T</sup> and PFR10<sup>T</sup> was menaquinone-7. Phylogenomic, phenotypic and genome analyses strongly indicated that chi10<sup>T</sup> and PFR10<sup>T</sup> could be two novel <i>Paenibacillus</i> species for which the names <i>Paenibacillus suaedae</i> sp. nov. (type strain chi10<sup>T</sup> = KACC 23258<sup>T</sup> = TBRC 17803<sup>T</sup>) and <i>Paenibacillus violae</i> sp. nov. (type strain PFR10<sup>T</sup> = KACC 23263<sup>T</sup> = TBRC 17804<sup>T</sup>) are proposed, respectively.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006542","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
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Abstract
Two novel Gram-positive strains, chi10T and PFR10T, were isolated from the roots of Suaeda japonica Makino and Viola mandshurica W. Becker, respectively. The strains are facultatively aerobic, rod-shaped, motile via peritrichous flagella and endospore-forming. Strains chi10T and PFR10T showed the highest 16S rRNA gene sequence similarity to Paenibacillus alvei DSM 29T (99.0%) and Paenibacillus planticolens LMG 31457T (99.3%). Furthermore, the phylogenetic and phylogenomic analyses demonstrated that strains chi10T and PFR10T were closely related to the genus Paenibacillus. The digital DNA-DNA hybridization (dDDH) values for strains used in the phylogenetic analysis and strain chi10T ranged from 19.6 to 28.4%, while for strain PFR10T, the values ranged from 19.8 to 53.5%, according to the Genome-to-Genome Distance Calculator 3.0, which were all less than 70%. Additionally, the dDDH value between strains chi10T and PFR10T was 22.0%, confirming that they are different species of the genus Paenibacillus. The average nucleotide identity between chi10T and PFR10T and other species of the genus Paenibacillus were 68.4-84.7% and 67.8-93.6%, respectively. In addition, the major polar lipids of chi10T and PFR10T consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acid profile of strain chi10T was iso-C15 : 0 and anteiso-C15 : 0, whereas the major fatty acid profile of strain PFR10T was iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. The sole respiratory quinone in strains chi10T and PFR10T was menaquinone-7. Phylogenomic, phenotypic and genome analyses strongly indicated that chi10T and PFR10T could be two novel Paenibacillus species for which the names Paenibacillus suaedae sp. nov. (type strain chi10T = KACC 23258T = TBRC 17803T) and Paenibacillus violae sp. nov. (type strain PFR10T = KACC 23263T = TBRC 17804T) are proposed, respectively.
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