NBF2, an Algal Fiber-Rich Formula, Reverses Diabetic Dyslipidemia and Hyperglycemia In Vivo.

IF 5.6 2区生物学

International Journal of Molecular Sciences Pub Date : 2024-10-09 DOI:10.3390/ijms251910828

Nlandu Roger Ngatu, Akram Hossain, Nao Maruo, Steeve Akumwami, Asadur Md Rahman, Masamitsu Eitoku, Kanae Kanda, Akira Nishiyama, Narufumi Suganuma, Tomohiro Hirao

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Abstract

Ulva prolifera, known as Aonori in Japan, is an edible alga species that is mass-cultivated in Japan. Supplementation with Aonori-derived biomaterials has been reported to enhance metabolic health in previous studies. This was an experimental study that evaluated the metabolic health effects of NBF2, a formula made of algal and junos Tanaka citrus-derived biomaterials, on obesity and type 2 diabetes (T2DM). We used 18 obese and hyperglycemic Otsuka Long-Evans Tokushima Fatty (OLETF) rats that were assigned randomly to three groups of six animals: a high-dose NBF2 drink (20 mg/kg) group, a low-dose (10 mg/kg) NBF2 drink group and the control group that received 2 mL of tap water daily for a total of six weeks. We also used eight LETO rats as the normal control group. In addition to the glucose tolerance test (OGTT), ELISA and real-time PCR assays were performed. High-dose and lowdose NBF2 improved insulin sensitivity, as well as glycemic and lipid profiles, as compared with control rats. The OGTT showed that both NBF2 groups and LETO rats had normalized glycemia by the 90-min time-point. NBF2 up-regulated PPARα/γ-mRNA and Sirt2-mRNA gene expressions in BAT and improved the blood pressure profile. These findings suggest that the NBF2 formula, which activates PPAR-α/γ mRNA and Sirt2-mRNA, may reverse dyslipidemia and hyperglycemia in T2DM.

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富含海藻纤维的配方奶粉 NBF2 可在体内逆转糖尿病血脂异常和高血糖。

莼菜在日本被称为 "Aonori"，是一种可食用的藻类，在日本被大量种植。据报道，在以前的研究中，补充青海藻提取的生物材料可增强代谢健康。这是一项实验研究，旨在评估 NBF2（一种由藻类和田中杜松柑橘提取的生物材料制成的配方）对肥胖和 2 型糖尿病（T2DM）的代谢健康影响。我们使用了 18 只肥胖和高血糖的大冢长伊万德岛脂肪大鼠（OLETF），将其随机分配到三组，每组六只：高剂量 NBF2 饮料（20 毫克/千克）组、低剂量（10 毫克/千克）NBF2 饮料组和每天饮用 2 毫升自来水的对照组，共六周。我们还使用了八只 LETO 大鼠作为正常对照组。除葡萄糖耐量试验（OGTT）外，还进行了 ELISA 和实时 PCR 检测。与对照组大鼠相比，高剂量和低剂量 NBF2 均能改善胰岛素敏感性以及血糖和血脂状况。OGTT显示，NBF2组和LETO组大鼠在90分钟的时间点上血糖均已恢复正常。NBF2 上调了 BAT 中 PPARα/γ-mRNA 和 Sirt2-mRNA 的基因表达，并改善了血压状况。这些发现表明，激活 PPAR-α/γ mRNA 和 Sirt2-mRNA 的 NBF2 配方可逆转 T2DM 中的血脂异常和高血糖。

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来源期刊

International Journal of Molecular Sciences 化学-化学综合

自引率

10.70%

发文量

13472

审稿时长

1.7 months

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).