The presence of yeasts and bacteria in free-living amoebae isolated from COVID-19 patients: concern for secondary infections.

IF 2.2 4区医学 Q3 ENVIRONMENTAL SCIENCES

International Journal of Environmental Health Research Pub Date : 2024-10-20 DOI:10.1080/09603123.2024.2409830

Zahra Mirabedini, Maryam Niyyati, Hanieh Mohammad Rahimi, Sara Soleimani Jevinani, Marziye Fatemi, Mohammad Tanhaei, Seyed Reza Mohebbi, Abbas Yadegar, Sara Abolghasemi, Zahra Arab Mazar, Hamed Mirjalali

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引用次数: 0

Abstract

This study aimed to investigate the presence of SARS-CoV-2, yeasts, and bacteria in isolated free-living amoeba (FLA) from COVID-19 patients. Nasopharyngeal swabs (n = 60) were obtained from COVID-19 patients. After cultivation, morphological characterization, and RNA/DNA extraction, the presence of selected microorganisms was investigated. From 60 COVID-19 samples, 18 (30%) were positive for FLA. Acanthamoeba sp. Naegleria australiensis, Tetramitus sp. and Vermamoeba vermiformis were characterized in 12 (80%), 1 (6.66%), 2 (13.33%), and 7 (38.88%) of samples, respectively. SARS-CoV-2 RNA was not detected in FLA. Candida albicans, C. tropicalis, and C. parapsilosis were detected in (11/18; 61.11%), (3/18; 16.67%), and (3/18; 16.67%) of samples, respectively. Geotrichum candidum was detected in 10/18 (55.55%) of samples. Streptococcus spp. and Staphylococcus spp. were identified in 16/18 (88.88%) and 3/18 (16.67%), respectively. The presence of yeasts and bacteria signifies the possible role of FLA in distribution of secondary infections in susceptible patients.

查看原文本刊更多论文

从 COVID-19 患者体内分离出的自由生活变形虫中存在酵母菌和细菌：担心继发感染。

本研究旨在调查从 COVID-19 患者体内分离出的自由生活阿米巴（FLA）中是否存在 SARS-CoV-2、酵母菌和细菌。研究人员从 COVID-19 患者身上采集了鼻咽拭子（n = 60）。经过培养、形态鉴定和 RNA/DNA 提取后，对所选微生物的存在情况进行了调查。在 60 份 COVID-19 样本中，18 份（30%）对 FLA 呈阳性。在 12 个样本（80%）、1 个样本（6.66%）、2 个样本（13.33%）和 7 个样本（38.88%）中分别鉴定出了棘阿米巴、澳大利亚奈格勒氏虫、四螨虫和疣状蛭。在 FLA 中未检测到 SARS-CoV-2 RNA。白念珠菌、热带念珠菌和副丝状念珠菌分别在（11/18；61.11%）、（3/18；16.67%）和（3/18；16.67%）个样本中检测到。10/18（55.55%）个样本中检测到念珠菌。在 16/18 个样本（88.88%）和 3/18 个样本（16.67%）中分别检测到链球菌属和葡萄球菌属。酵母菌和细菌的存在表明，FLA 在易感患者继发感染的分布中可能扮演了重要角色。

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来源期刊

International Journal of Environmental Health Research 医学-公共卫生、环境卫生与职业卫生

CiteScore

6.70

自引率

3.10%

发文量

134

审稿时长

>12 weeks

期刊介绍： International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.