Distinct Clusters of HIV-1 CRF01_AE in Zhejiang, China: High-Risk Transmission Cluster 4 Requires Heightened Surveillance.

IF 2.9 3区医学 Q2 INFECTIOUS DISEASES

Infection and Drug Resistance Pub Date : 2024-10-11 eCollection Date: 2024-01-01 DOI:10.2147/IDR.S480192

Bohao Dai, Xiaorong Peng, Jia Sun, Xueling Zhu, Xiang Liu, Ye Xiong, Zhikai Wan, Dairong Xiang, Jiangjin Hui, Chenxi Ying, Huiting Liu, Biao Zhu

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引用次数: 0

Abstract

Background: HIV-1 CRF01_AE is becoming the predominant HIV-1 subtype among patients in China. The distribution and characteristics of transmission clusters of HIV-1 CRF01_AE in Zhejiang, Eastern China remains unclear. This study analyzed the epidemiologic characteristics and transmission clusters of HIV-1 CRF01_AE in Zhejiang.

Methods: Plasma samples obtained from 152 patients of HIV-1 CRF01_AE not undergoing ART were used to amplify HIV-1 pol and env gene. CRF01_AE drug resistance mutations (DRM) prevalence was analysed using Stanford University's HIV Drug Resistance Database. A phylogenetic tree was constructed using FastTree (version 2.1.11) based on the GTR nucleotide substitution model and visualized using Figtree (version 1.4.4) and The Interactive Tree of Life; the Chinese HIV Gene Sequence Data Platform was used to construct genetic transmission networks.

Results: Majority samples could be grouped into CRF01_AE transmission Clusters 1 (11.2%), 4 (64.5%), and 5 (7.2%). The CD4+ T-cell counts in Cluster 1, 4a, 4b are lower than 5 were 15, 38, 30, and 248 cells/mm³, respectively (P < 0.05). The high X4 tropism rates were 13.2%, 11.8%, 20.0%, and 0.0% in Clusters 1, 4a, 4b, and 5, respectively. DRM rates in Clusters 4a and 4b were 17.6%, and 25.45% respectively (P < 0.05), whereas they were 17.6% and 18.2% in Clusters 1 and 5, respectively. In total, 24 transmission genetic networks, comprising 72 sequences and 61 links, were discovered; of them, 61.2%, 11.7%, and 18.2% were from Clusters 4, 1, and 5, respectively (P < 0.05).

Conclusion: In Zhejiang, different CRF01_AE clusters displayed unique clinic features. Cluster 4, particularly Cluster 4b, was considered a high-risk transmission cluster. The surveillance of epidemiology of HIV-1 should be enhanced to minimize its transmission.

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中国浙江 HIV-1 CRF01_AE 的不同群集：高危传播群集 4 需要加强监测。

背景：HIV-1 CRF01_AE 正在成为中国患者中最主要的 HIV-1 亚型。HIV-1 CRF01_AE 在中国东部浙江地区的分布和传播集群特征尚不清楚。本研究分析了浙江地区 HIV-1 CRF01_AE 的流行病学特征和传播集群：方法：对152例未接受抗逆转录病毒治疗的HIV-1 CRF01_AE患者的血浆样本进行HIV-1 pol和env基因扩增。使用斯坦福大学的 HIV 耐药性数据库分析 CRF01_AE 耐药性突变（DRM）的发生率。使用FastTree（2.1.11版）基于GTR核苷酸替换模型构建系统发生树，并使用Figtree（1.4.4版）和交互式生命树进行可视化；使用中国HIV基因序列数据平台构建基因传播网络：大多数样本可归入CRF01_AE传播群1（11.2%）、4（64.5%）和5（7.2%）。群组 1、4a、4b 的 CD4+ T 细胞数低于群组 5，分别为 15、38、30 和 248 cells/mm3（P < 0.05）。群组 1、4a、4b 和 5 的 X4 滋养率分别为 13.2%、11.8%、20.0% 和 0.0%。群组 4a 和 4b 的 DRM 率分别为 17.6% 和 25.45% （P < 0.05），而群组 1 和 5 的 DRM 率分别为 17.6% 和 18.2%。共发现24个传播遗传网络，包括72个序列和61个链接，其中第4、1和5群分别占61.2%、11.7%和18.2%（P<0.05）：结论：在浙江，不同的 CRF01_AE 群显示出独特的临床特征。结论：在浙江，不同的 CRF01_AE 群显示出独特的临床特征，第 4 群，尤其是第 4b 群被认为是高危传播群。应加强对 HIV-1 流行病学的监测，以减少其传播。

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来源期刊

Infection and Drug Resistance Medicine-Pharmacology (medical)

CiteScore

5.60

自引率

7.70%

发文量

826

审稿时长

16 weeks

期刊介绍： About Journal Editors Peer Reviewers Articles Article Publishing Charges Aims and Scope Call For Papers ISSN: 1178-6973 Editor-in-Chief: Professor Suresh Antony An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.