Clinical relevance and frequency of cytoplasmic patterns observed in ANA-Hep-2: experience of Cairo University Hospitals.

IF 3.3 4区 医学 Q3 IMMUNOLOGY
Immunologic Research Pub Date : 2024-12-01 Epub Date: 2024-10-21 DOI:10.1007/s12026-024-09551-z
Fatma Hassan Abdelraouf, Omnia DeiaaEldin Soliman, Engy Mohammad El Khateeb, Aya Erfan Mostafa
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引用次数: 0

Abstract

Antinuclear antibodies (ANA) are the most common biomarkers observed in autoimmune diseases. Cytoplasmic staining patterns on ANA-Hep-2 are gaining recognition but with scanty information about their clinical and diagnostic role. The aim is to assess the frequency of cytoplasmic ANA patterns in autoimmune diseases, and to evaluate their possible associations with clinical diagnoses and autoantibodies. This observational cross-sectional study was conducted by examining and/or reviewing ANA by indirect immunofluorescence assay during a 13-month period. This was followed by testing the group of patients with a positive cytoplasmic staining pattern (n = 92) using the Microblot-Array ANA plus for the presence of 44 specific autoantibodies. Out of 2741 samples, 1791 (65.3%) tested negative, 845 (30.9%) tested positive nuclear staining patterns, 56 (2.0%) positive solitary cytoplasmic staining patterns, and 49 (1.8%) positive mixed nuclear and cytoplasmic patterns. Ninety-two cases (3.4% of the total cases) were analyzed using Microblot-Array ANA plus, with reticular as the most frequent cytoplasmic pattern, followed by dense fine speckled. The most frequently associated disease with reticular pattern was primary biliary cholangitis (28.9%), and the most frequently detected autoantibodies were against M2 (66.7%). The most frequently associated disease with dense fine speckled pattern was systemic lupus erythematosus (69.4%), and the most frequently detected autoantibodies were against nucleosome (57.7%) and ribosomal P0 (53.8%). This study highlights the significance of reporting cytoplasmic staining patterns and their importance in assessment of autoimmune diseases. Larger cohort studies on treatment naïve patients are recommended.

在 ANA-Hep-2 中观察到的细胞质模式的临床相关性和频率:开罗大学医院的经验。
抗核抗体(ANA)是自身免疫性疾病中最常见的生物标志物。ANA-Hep-2的细胞质染色模式正逐渐得到认可,但有关其临床和诊断作用的信息却很少。本研究旨在评估细胞质 ANA 模式在自身免疫性疾病中的出现频率,并评估其与临床诊断和自身抗体之间可能存在的关联。这项观察性横断面研究通过间接免疫荧光检测法对 ANA 进行检查和/或复查,为期 13 个月。随后,对细胞质染色模式呈阳性的一组患者(n = 92)使用微印迹阵列 ANA plus 检测 44 种特异性自身抗体。在 2741 份样本中,1791 份(65.3%)检测结果为阴性,845 份(30.9%)检测结果为细胞核染色模式阳性,56 份(2.0%)检测结果为单个细胞质染色模式阳性,49 份(1.8%)检测结果为细胞核和细胞质混合模式阳性。有 92 个病例(占病例总数的 3.4%)使用微印迹阵列 ANA plus 进行了分析,网状是最常见的细胞质模式,其次是致密细小斑点。最常出现网状模式的相关疾病是原发性胆汁性胆管炎(28.9%),最常检测到的自身抗体是针对 M2 的(66.7%)。最常出现密集细小斑点模式的相关疾病是系统性红斑狼疮(69.4%),最常检测到的自身抗体是针对核糖体(57.7%)和核糖体 P0(53.8%)的自身抗体。这项研究强调了报告细胞质染色模式的意义及其在评估自身免疫性疾病中的重要性。建议对未接受治疗的患者进行更大规模的队列研究。
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来源期刊
Immunologic Research
Immunologic Research 医学-免疫学
CiteScore
6.90
自引率
0.00%
发文量
83
审稿时长
6-12 weeks
期刊介绍: IMMUNOLOGIC RESEARCH represents a unique medium for the presentation, interpretation, and clarification of complex scientific data. Information is presented in the form of interpretive synthesis reviews, original research articles, symposia, editorials, and theoretical essays. The scope of coverage extends to cellular immunology, immunogenetics, molecular and structural immunology, immunoregulation and autoimmunity, immunopathology, tumor immunology, host defense and microbial immunity, including viral immunology, immunohematology, mucosal immunity, complement, transplantation immunology, clinical immunology, neuroimmunology, immunoendocrinology, immunotoxicology, translational immunology, and history of immunology.
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