Prevalence and Genetic Diversity of Entamoeba and Cryptosporidium in Pigs and Wild Boars in Central and Southern Vietnam: Implications for Zoonotic Risks and Surveillance.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Le Chi Cao, Devika Muraleedharan, Tran Thi Giang, Vo Minh Tiep, Ngo Thi Minh Chau, Ton Nu Phuong Anh, Le Nguyen Nhat Ha, Nguyen Thi Thu Hoai, Truong Nhat My, Awatef El Moussi, Nourhane Hafza, Le Huu Song, Thirumalaisamy P Velavan
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引用次数: 0

Abstract

Background: Parasites of Entamoeba and Cryptosporidium genera, prevalent among various vertebrates such as humans and pigs, pose a zoonotic threat as common protozoan pathogens. This study investigated the prevalence and genetic diversity of Entamoeba and Cryptosporidium species in pigs and wild boars across central and southern Vietnam, to ascertain parasite transmission dynamics. Methods: A total of 113 independent stool samples from 77 pigs and 36 wild boars were analyzed using PCR-based molecular methodologies to detect the presence of Entamoeba spp. and Cryptosporidium spp. The identified species were further characterized through Sanger sequencing, and phylogenetic relationships were analyzed. Results: The study revealed a high prevalence of Entamoeba spp. (62%, n = 70/113) and Cryptosporidium spp. (31%, n = 35/113). Entamoeba suis (57%, n = 40) was predominant, followed by Entamoeba polecki (40%, n = 40) and Entamoeba hartmanni (3%, n = 2). Among Cryptosporidium species, Cryptosporidium scrofarum (89%, n = 31) was the most common, followed by Cryptosporidium suis (11%, n = 4). Wild boars exhibited a higher prevalence of Entamoeba infection compared with domestic pigs (p = 0.019). Conclusions: The study highlights a high prevalence of Entamoeba and Cryptosporidium, suggesting a potential for zoonotic transmission in Vietnam. Further investigations are necessary to determine the extent to which these parasites in pigs and wild boars contribute to the burden in the human population.

越南中部和南部猪和野猪中恩他莫拉菌和隐孢子虫的流行率和遗传多样性:对人畜共患病风险和监测的影响。
背景:恩塔米巴和隐孢子虫属寄生虫在人和猪等各种脊椎动物中普遍存在,作为常见的原生动物病原体,对人畜共患病构成威胁。本研究调查了越南中部和南部的猪和野猪中恩塔米巴虫和隐孢子虫的流行情况和遗传多样性,以确定寄生虫的传播动态。研究方法使用基于 PCR 的分子方法对 77 头猪和 36 头野猪的 113 份独立粪便样本进行分析,以检测是否存在恩塔米巴属和隐孢子虫属。 通过桑格测序对确定的物种进一步定性,并分析其系统发育关系。结果研究显示,恩塔米巴属(62%,n = 70/113)和隐孢子虫属(31%,n = 35/113)的流行率很高。其中以苏氏恩塔米巴虫(57%,n = 40)为主,其次是杆状恩塔米巴虫(40%,n = 40)和哈特曼恩塔米巴虫(3%,n = 2)。在隐孢子虫中,最常见的是瘰疬隐孢子虫(89%,n = 31),其次是猪隐孢子虫(11%,n = 4)。与家猪相比,野猪的恩塔米巴感染率更高(p = 0.019)。结论这项研究表明,越南的恩塔米巴虫和隐孢子虫感染率很高,这表明越南可能存在人畜共患病传播。有必要开展进一步调查,以确定猪和野猪体内的这些寄生虫在多大程度上加重了人类的负担。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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