Atrazine dependence in cultivated fungal communities.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Gessyca Fernanda da Silva, Jose Alejandro Morales Gomez, João Victor Fonseca Moreira, Giulio Mendes Braatz, Rafaella Costa Bonugli-Santos
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引用次数: 0

Abstract

The isolation and study of fungi within specific contexts yield valuable insights into the intricate relationships between fungi and ecosystems. Unlike culture-independent approaches, cultivation methods are advantageous in this context because they provide standardized replicates, specific species isolation, and easy sampling. This study aimed to understand the ecological process using a microcosm system with pesticide concentrations similar to those found in the soil, in contrast to high doses, from the isolation of the enriched community. The atrazine concentrations used were 0.02 mg/kg (control treatment), 300 ng/kg (treatment 1), and 3000 ng/kg (treatment 2), using a 28-day microcosm system. Ultimately, the isolation resulted in 561 fungi classified into 76 morphospecies. The Ascomycota phylum was prevalent, with Purpureocillium, Aspergillus, and Trichoderma being consistently isolated, denoting robust and persistent genera. Diversity analyses showed that the control microcosms displayed more distinct fungal morphospecies, suggesting the influence of atrazine on fungal communities. Treatment 2 (higher atrazine concentration) showed a structure comparable to that of the control, whereas treatment 1 (lower atrazine concentration) differed significantly, indicating that atrazine concentration impacted community variance. Higher atrazine addition subtly altered ligninolytic fungal community dynamics, implying its potential for pesticide degradation. Finally, variations in atrazine concentrations triggered diverse community responses over time, shedding light on fungal resilience and adaptive strategies against pesticides.

栽培真菌群落对阿特拉津的依赖性。
在特定环境中分离真菌并对其进行研究,可为了解真菌与生态系统之间错综复杂的关系提供宝贵的信息。与不依赖培养的方法不同,培养方法在这方面具有优势,因为它们提供了标准化的重复、特定物种的分离和简便的取样。本研究旨在利用微宇宙系统了解生态过程,该系统中的农药浓度与土壤中的浓度相似,而不是高剂量,从富集群落中分离出来。使用的阿特拉津浓度分别为 0.02 毫克/千克(对照处理)、300 纳克/千克(处理 1)和 3000 纳克/千克(处理 2),微生态系统使用期为 28 天。最终分离出 561 种真菌,分为 76 个形态种。子囊菌门是主要菌门,其中紫云英属、曲霉属和毛霉属一直被分离出来,表明这些菌属很强健且具有持久性。多样性分析表明,对照微生态系统显示出更多不同的真菌形态物种,这表明阿特拉津对真菌群落有影响。处理 2(阿特拉津浓度较高)显示出与对照组相当的结构,而处理 1(阿特拉津浓度较低)则差异显著,表明阿特拉津浓度对群落差异有影响。较高的阿特拉津添加量微妙地改变了木质素分解真菌群落的动态,这意味着阿特拉津具有降解农药的潜力。最后,随着时间的推移,阿特拉津浓度的变化引发了不同的群落反应,揭示了真菌的恢复能力和对农药的适应策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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