Assessing inter-ocular fixational eye movements throughout the lifespan.

Abstract

This study aims to assess fixational eye movements (FEMs) obtained under binocular and monocular viewing in normal individuals across different age groups. We recruited 68 healthy participants divided into Group 1 (children, 3-9 years, n = 20), Group 2 (adolescents, 10-19 years, n = 26), and Group 3 (adults, 20-73 years, n = 22). FEMs were collected using a high-resolution video-based tracker under 3 viewing conditions: binocular viewing (BV), monocular viewing right eye (MV_RE), and monocular viewing left eye (MV_LE). We quantified fixation stability, the frequency, amplitude, and disconjugacy of fixational saccades, and inter-saccadic drift velocity in BV, MV_RE, and MV_LE. We also computed inter-ocular fixation stability under binocular viewing and monocular viewing in the 3 groups. Fixation instability (FI) and fixational saccade amplitudes were higher in Group 1 than in Group 3 whereas inter-saccadic drifts were increased in Group 3. Vergence stability was greater in binocular viewing than in monocular viewing likely due to binocular summation in all groups. However, the fixational saccade amplitude and drift velocity of the right and left eye did not significantly differ across different viewing conditions within each group. Interestingly, the inter-ocular fixation stability ratio and vergence stability showed no significant differences between the groups. In conclusion, FEMs differ across age groups but inter-ocular FEMs are immune to the effects of age and can be a valuable parameter while evaluating FEM abnormalities in diseases like amblyopia.