Envelope Protein in Differential Serodiagnosis of Dengue, Zika, and Chikungunya Viruses: A Systematic Review.

IF 2.9 4区医学 Q3 CHEMISTRY, MEDICINAL

Current topics in medicinal chemistry Pub Date : 2024-10-18 DOI:10.2174/0115680266348828241008214528

Amauri Donadon Leal Junior, Fernando Américo Jorge, Franciele Abigail Vilugron Rodrigues-Vendramini, Pollyanna Cristina Vincenzi Conrado, Deborah de Castro Moreira, Rafaela Daleffe Pepino, Isis Regina Grenier Capoci, Patrícia de Souza Bonfim-Mendonça, Luciana Dias Ghiraldi Lopes, Dennis Armando Bertolini, Izabel Galhardo Demarchi, Jorge Juarez Vieira Teixeira, Érika Seki Kioshima

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引用次数: 0

Abstract

Objectives: This systematic review was conducted to evaluate the applicability of the envelope (E) protein in the diagnosis of arboviruses.

Methods: This review was performed in accordance with the PRISMA statement. Five databases were explored (PubMed, Web of Science, Scopus, EMBASE, and IEDB). The inclusion and exclusion criteria were applied to study eligibility. After data extraction, the risk of bias and evidence certainty were evaluated according to QUADAS and GRADE assessments, respectively.

Results: Eleven studies were included. A total of 11 studies were included in the review. ELISA was the most frequently utilized technique, with two studies employing it for antigen detection and nine for antibodies. The E protein was used as a whole protein, heterologous protein, and peptides. The diagnostic metrics were enhanced by optimizations on techniques, such as antibody capture, competitors, and nanosensors. Monoclonal antibodies showed improved specificity, including in coinfected samples. Seven studies demonstrated a minimal risk of bias, and the evidence certainty was considered moderate for dengue diagnosis.

Conclusions: The E protein was successfully employed in different immunological assays with large-scale strategies, enhancing the applicability potential for differential arboviruses' diagnosis. Furthermore, both the antigen design and the implementation of innovative methodologies will have a substantial impact on the quality of the new tests. The PROSPERO protocol related to this work: CRD42021265243.

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登革热、寨卡和基孔肯雅病毒血清诊断差异中的包膜蛋白：系统综述。

目的：本系统综述旨在评估包膜蛋白（E）在虫媒病毒诊断中的适用性：本系统综述旨在评估包膜（E）蛋白在虫媒病毒诊断中的适用性：本综述根据 PRISMA 声明进行。方法：本综述按照 PRISMA 声明进行，浏览了五个数据库（PubMed、Web of Science、Scopus、EMBASE 和 IEDB）。纳入和排除标准适用于研究资格。数据提取后，分别根据 QUADAS 和 GRADE 评估方法对偏倚风险和证据确定性进行了评估：结果：共纳入 11 项研究。共有 11 项研究被纳入综述。酶联免疫吸附法是最常用的技术，有两项研究使用该方法检测抗原，九项研究使用该方法检测抗体。E 蛋白被用作全蛋白、异源蛋白和肽。抗体捕获、竞争者和纳米传感器等技术的优化提高了诊断指标。单克隆抗体的特异性有所提高，包括在合并感染样本中。七项研究显示偏倚风险极低，登革热诊断的证据确定性为中等：结论：E 蛋白被成功地应用于不同的免疫测定中，并采用了大规模策略，从而提高了其在不同虫媒病毒诊断中的应用潜力。此外，抗原设计和创新方法的实施都将对新检测方法的质量产生重大影响。与本研究相关的 PROSPERO 协议：CRD42021265243。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current topics in medicinal chemistry 医学-医药化学

CiteScore

6.40

自引率

2.90%

发文量

186

审稿时长

3-8 weeks

期刊介绍： Current Topics in Medicinal Chemistry is a forum for the review of areas of keen and topical interest to medicinal chemists and others in the allied disciplines. Each issue is solely devoted to a specific topic, containing six to nine reviews, which provide the reader a comprehensive survey of that area. A Guest Editor who is an expert in the topic under review, will assemble each issue. The scope of Current Topics in Medicinal Chemistry will cover all areas of medicinal chemistry, including current developments in rational drug design, synthetic chemistry, bioorganic chemistry, high-throughput screening, combinatorial chemistry, compound diversity measurements, drug absorption, drug distribution, metabolism, new and emerging drug targets, natural products, pharmacogenomics, and structure-activity relationships. Medicinal chemistry is a rapidly maturing discipline. The study of how structure and function are related is absolutely essential to understanding the molecular basis of life. Current Topics in Medicinal Chemistry aims to contribute to the growth of scientific knowledge and insight, and facilitate the discovery and development of new therapeutic agents to treat debilitating human disorders. The journal is essential for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important advances.