A lipidomic approach towards identifying the effects of fragrance hydroperoxides on keratinocytes.

IF 4.8 1区 医学 Q2 ALLERGY
Aaron I Moore, Ana S P Moreira, Inês M S Guerra, Laura Goracci, Pedro Domingues, Tânia Melo, M Rosário Domingues, Niamh M O'Boyle
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引用次数: 0

Abstract

Background: Limonene and linalool are used in cosmetic products for their floral scents, but their oxidation products are strong contact allergens whose mechanisms of action are still not fully understood.

Objectives: The effects of limonene hydroperoxide (Lim-2-OOH) and linalool hydroperoxides (Lin-6/7-OOH) on the lipid profile of a human keratinocyte cell line (HaCaT) were evaluated. 2,4-Dinitrofluorobenzene (DNFB) was also included.

Methods: Lim-2-OOH and Lin-6/7-OOH were synthesised according to previous methods. HaCaT cells were treated with allergens (10 μM) for 24 h and the cellular lipid extracts were analysed by C18 liquid chromatography with tandem mass spectrometry (LC-MS/MS). Data analysis was performed using Lipostar software. Statistical analysis was carried out using Metaboanalyst and R software.

Results: All three sensitisers used caused significant changes in the lipidome of HaCaT cells in a similar trend. There was an upregulation in several plasmanyl/plasmenyl phospholipids (O-/P-phosphatidylcholines [PC] and O-/P-phosphatidylethanolamines [PE]), sphingolipids (HexCer) and triacylglycerol lipid species, and a decrease in some polyunsaturated fatty acids-containing phospholipid (PE and PC) species suggesting oxidative stress and inflammation.

Conclusions: This study is the first to evaluate the plasticity of the HaCaT cell lipidome in response to allylic hydroperoxide allergens Lim-2-OOH and Lin-6/7-OOH, together with the experimental contact allergen DNFB. These allergens are able to upregulate and downregulate certain lipid classes to a varying degree.

通过脂质组学方法确定香料氢过氧化物对角质细胞的影响。
背景:柠檬烯和芳樟醇因其花香而被用于化妆品中,但它们的氧化产物是强烈的接触性过敏原,其作用机制尚未完全明了:评估了柠檬烯氢过氧化物(Lim-2-OOH)和芳樟醇氢过氧化物(Lin-6/7-OOH)对人类角质细胞系(HaCaT)脂质谱的影响。方法:根据以前的方法合成了 Lim-2-OOH 和 Lin-6/7-OOH。用过敏原(10 μM)处理 HaCaT 细胞 24 小时,然后用 C18 液相色谱-串联质谱法(LC-MS/MS)分析细胞脂质提取物。数据分析采用 Lipostar 软件进行。使用 Metaboanalyst 和 R 软件进行统计分析:结果:所使用的三种敏化剂都会导致 HaCaT 细胞脂质体发生显著变化,且变化趋势相似。几种plasmanyl/plasmmenyl磷脂(O-/P-磷脂酰胆碱[PC]和O-/P-磷脂酰乙醇胺[PE])、鞘脂(HexCer)和三酰甘油脂类上调,而一些含多不饱和脂肪酸的磷脂(PE和PC)类下降,表明存在氧化应激和炎症:本研究首次评估了 HaCaT 细胞脂质体对烯丙基过氧化氢过敏原 Lim-2-OOH 和 Lin-6/7-OOH 以及实验性接触过敏原 DNFB 的可塑性。这些过敏原能在不同程度上上调或下调某些脂质类别。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Contact Dermatitis
Contact Dermatitis 医学-过敏
CiteScore
4.60
自引率
30.90%
发文量
227
审稿时长
4-8 weeks
期刊介绍: Contact Dermatitis is designed primarily as a journal for clinicians who are interested in various aspects of environmental dermatitis. This includes both allergic and irritant (toxic) types of contact dermatitis, occupational (industrial) dermatitis and consumers" dermatitis from such products as cosmetics and toiletries. The journal aims at promoting and maintaining communication among dermatologists, industrial physicians, allergists and clinical immunologists, as well as chemists and research workers involved in industry and the production of consumer goods. Papers are invited on clinical observations, diagnosis and methods of investigation of patients, therapeutic measures, organisation and legislation relating to the control of occupational and consumers".
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