Regulation of Curcumin on Follicle Initiation Rate in Diminished Ovarian Reserve.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Combinatorial chemistry & high throughput screening Pub Date : 2024-10-14 DOI:10.2174/0113862073327087240926065629

Wanjing Li, Jinbang Xu, Jingyi Wang, Junxin Zhang, Disi Deng

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引用次数: 0

Abstract

Aim: To study the mechanism by which curcumin regulates ovarian primordial follicle initiation in rats with triptolide-induced diminished ovarian reserve (DOR).

Methods: An in vitro gelatin sponge culture was performed on 3-day-old rat ovaries. After the establishment of the DOR model with triptolide, curcumin was administered for 3 days. Histological analysis and follicle counts were performed using H&E staining. ELISA detection of ovarian hormones in the culture medium (E2, FSH and LH), western blotting and Q-PCR for protein and mRNA expression (LTCONS-00011173, TGF-β1, Smad1, AMH, PTEN and GDF-9).

Results: Ovarian primordial and growing follicles increased significantly after curcumin intervention (p < 0.05), FSH/LH and E2 levels were increased significantly (p < 0.05). Curcumin also significantly decreased the expression of LTCONS-00011173. Meanwhile, curcumin increased the expression of TGF-β, AMH, and GDF-9 (p < 0.05). In addition, curcumin increased Smad1 gene expression and protein phosphorylation in the ovary on the one hand (p < 0.05), but inhibited Smad1 and p-Smad1 protein expression on the other hand (p < 0.05). Moreover, curcumin decreased PTEN protein and mRNA expression (p < 0.05).

Conclusion: Curcumin activates primordial follicles in DOR model rats through TGF-β1 and downstream AMH signaling pathways and may limit follicle exhaustion through LncRNA.

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姜黄素对卵巢储备功能减退时卵泡启动率的调节作用

目的：研究姜黄素调节三苯氧胺诱导的卵巢储备功能减退（DOR）大鼠卵巢原始卵泡启动的机制：方法：对3日龄大鼠卵巢进行体外明胶海绵培养。方法：对 3 天大的大鼠卵巢进行体外明胶海绵培养。使用 H&E 染色法进行组织学分析和卵泡计数。ELISA检测培养基中的卵巢激素（E2、FSH和LH），Western印迹和Q-PCR检测蛋白和mRNA表达（LTCONS-00011173、TGF-β1、Smad1、AMH、PTEN和GDF-9）：结果：姜黄素干预后，卵巢原始卵泡和生长卵泡明显增加（P < 0.05），FSH/LH和E2水平明显增加（P < 0.05）。姜黄素还明显降低了LTCONS-00011173的表达。同时，姜黄素增加了 TGF-β、AMH 和 GDF-9 的表达（p < 0.05）。此外，姜黄素一方面增加了卵巢中Smad1基因的表达和蛋白磷酸化（p < 0.05），另一方面抑制了Smad1和p-Smad1蛋白的表达（p < 0.05）。此外，姜黄素还降低了PTEN蛋白和mRNA的表达（p < 0.05）：姜黄素通过TGF-β1和下游AMH信号通路激活DOR模型大鼠的原始卵泡，并可通过LncRNA限制卵泡衰竭。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.