Comprehensive mapping of sensory and sympathetic innervation of the developing kidney.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2024-10-22 Epub Date: 2024-10-15 DOI:10.1016/j.celrep.2024.114860

Pierre-Emmanuel Y N'Guetta, Sarah R McLarnon, Adrien Tassou, Matan Geron, Sepenta Shirvan, Rose Z Hill, Grégory Scherrer, Lori L O'Brien

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Abstract

The kidneys act as finely tuned sensors to maintain physiological homeostasis. Both sympathetic and sensory nerves modulate kidney function through precise neural control. However, how the kidneys are innervated during development to support function remains elusive. Using light-sheet and confocal microscopy, we generated anatomical maps of kidney innervation across development. Kidney innervation commences on embryonic day 13.5 (E13.5) as network growth aligns with arterial differentiation. Fibers are synapsin I⁺, highlighting ongoing axonogenesis and potential signaling crosstalk. By E17.5, axons associate with nephrons, and the network continues to expand postnatally. CGRP⁺, substance P⁺, TRPV1⁺, and PIEZO2⁺ sensory fibers and TH⁺ sympathetic fibers innervate the developing kidney. TH⁺ and PIEZO2⁺ axons similarly innervate the human kidney, following the arterial tree to reach targets. Retrograde tracing revealed the primary dorsal root ganglia, T10-L2, from which sensory neurons project to the kidneys. Together, our findings elucidate the temporality and neuronal diversity of kidney innervation.

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全面绘制发育中肾脏的感觉和交感神经支配图。

肾脏是维持生理平衡的精密传感器。交感神经和感觉神经通过精确的神经控制调节肾脏功能。然而，肾脏在发育过程中如何接受神经支配以支持其功能仍是一个谜。我们利用光片和共聚焦显微镜绘制了肾脏神经支配在整个发育过程中的解剖图。肾脏神经支配始于胚胎第 13.5 天（E13.5），因为网络生长与动脉分化一致。纤维突触素 I+，突显了正在进行的轴突生成和潜在的信号串扰。到 E17.5 期，轴突与肾小球结合，网络在出生后继续扩展。CGRP+、P物质+、TRPV1+和PIEZO2+感觉纤维以及TH+交感纤维支配着发育中的肾脏。TH+和PIEZO2+轴突同样支配人类肾脏，并沿着动脉树到达目标。逆行追踪发现了初级背根神经节 T10-L2，感觉神经元就是从这里投射到肾脏的。我们的发现共同阐明了肾脏神经支配的时间性和神经元多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.