Fine mapping and identification of the downy mildew resistance gene BoDMR2 in Cabbage (Brassica oleracea L. var. capitata).

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Yuankang Wu, Bin Zhang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Xilin Hou, Yangyong Zhang
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引用次数: 0

Abstract

Background: Cabbage (Brassica oleracea L. var. capitata) is an important crop within the Brassica oleracea species and is extensively cultivated worldwide. In recent years, outbreaks of downy mildew caused by Hyaloperonospora parasitica have resulted in substantial losses in cabbage production. Despite this, there have been limited studies on genes associated with resistance to downy mildew in cabbage.

Results: This study identified sister lines exhibiting significant differences in disease resistance and susceptibility. Using bulked segregant analysis followed by sequencing (BSA-seq) and linkage analysis, the cabbage resistance locus BoDMR2 was accurately mapped to an approximately 300 kb interval on chromosome 7. Among the candidate genes identified, several single nucleotide polymorphisms (SNPs) and a 3-bp insertion were found within the conserved domain of the Bo7g117810 gene, encoding a leucine-rich repeat domain protein, in susceptible genotypes. Additionally, real-time quantitative polymerase chain reaction (RT‒qPCR) analysis revealed that the expression level of Bo7g117810 in resistant specimens was 2.5-fold higher than that in susceptible specimens. An insertion‒deletion (InDel) marker was designed based on the identified insertion in susceptible materials, facilitating the identification and selection of downy mildew-resistant cabbage cultivars.

Conclusions: This study identifies Bo7g117810 as a potential candidate gene associated with adult-stage resistance to downy mildew in cabbage, supported by observed differences in gene sequence and expression levels. Furthermore, the development of an InDel marker I1-3, based on its mutation, provides valuable resources for breeding resistant cabbage cultivars.

甘蓝(Brassica oleracea L. var. capitata)抗霜霉病基因 BoDMR2 的精细图谱绘制与鉴定。
背景:卷心菜(Brassica oleracea L. var. capitata)是芸苔属(Brassica oleracea)中的一种重要作物,在世界各地广泛种植。近年来,由 Hyaloperonospora parasitica 引起的霜霉病的爆发导致了甘蓝生产的巨大损失。尽管如此,有关甘蓝抗霜霉病基因的研究还很有限:结果:这项研究确定了在抗病性和易感性方面表现出显著差异的姊妹品系。通过大量测序后的分离分析(BSA-seq)和连锁分析,甘蓝抗性基因座 BoDMR2 被精确定位到 7 号染色体上大约 300 kb 的区间。在确定的候选基因中,在易感基因型中发现了编码富亮氨酸重复结构域蛋白的 Bo7g117810 基因保守结构域内的几个单核苷酸多态性(SNPs)和一个 3-bp 插入。此外,实时定量聚合酶链反应(RT-qPCR)分析表明,抗性样本中 Bo7g117810 的表达水平是易感样本的 2.5 倍。根据在易感材料中发现的插入基因,设计了一个插入-缺失(InDel)标记,有助于抗霜霉病甘蓝栽培品种的鉴定和选育:结论:本研究通过观察到的基因序列和表达水平差异,确定了 Bo7g117810 是与甘蓝成株期抗霜霉病相关的潜在候选基因。此外,基于其突变开发的 InDel 标记 I1-3 为培育抗性甘蓝栽培品种提供了宝贵的资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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