Biotechnological potential of silver nanoparticles synthesized by green method to control phytopathogenic bacteria: contributions from a proteomic analysis.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Ivonaldo Reis Santos, Daiane Gonzaga Ribeiro, Pollyana da Nóbrega Mendes, Wagner Fontes, Isabelle Souza Luz, Luciano Paulino Silva, Angela Mehta
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引用次数: 0

Abstract

Silver nanoparticles (AgNPs) synthesized through green synthesis routes are widely used as antimicrobial agents due to their advantages such as biocompatibility, stability, sustainability, speed and cost-effectiveness. Although AgNPs appear to be more potent than silver ions, the mechanisms related to their antibacterial activity are not yet fully understood. The most common proposed mechanism of AgNPs' toxicity so far is the release of silver ions and/or specific functions of the particles. In this context, the present study aimed to investigate the mechanisms of action of AgNPs synthesized using noni fruit peels (Morinda citrifolia) against the phytopathogen Xanthomonas campestris pv. campestris (Xcc) through proteomics. Xcc was treated with AgNPs (32 µM), AgNO3 (32 µM), or received no treatment (Ctrl - control condition), and its proteomic response was comprehensively characterized to elucidate the antimicrobial mechanisms of AgNPs in the phytopathogenic microorganism. A total of 352 differentially abundant proteins were identified. Most proteins were regulated in the AgNPs × Ctrl and AgNPs × AgNO3 comparisons/conditions. When Xcc treated with 32 µM AgNPs were compared to controls, the results showed 134 differentially abundant proteins, including 107 increased and 27 decreased proteins. In contrast, when Xcc treated with 32 µM AgNO3 were compared to Ctrl, the results showed only 14 differentially abundant proteins, including 10 increased proteins and 4 decreased proteins. Finally, when Xcc treated with 32 µM AgNPs were compared to Xcc treated with 32 µM AgNO3, the results showed 204 differentially abundant proteins, including 75 increased proteins and 129 decreased proteins. Gene ontology enrichment analysis revealed that most of the increased proteins were involved in important biological processes such as metal ion homeostasis, detoxification, membrane organization, metabolic processes related to amino acids and carbohydrates, lipid metabolic processes, proteolysis, transmembrane transport, and others. The AgNPs used in this study demonstrated effective antimicrobial activity against the phytopathogenic bacteria Xcc. Furthermore, the obtained results contribute to a better understanding of the mechanisms of action of AgNPs in Xcc and may aid in the development of strategies to control Xcc in brassica.

用绿色方法合成的银纳米粒子在控制植物病原菌方面的生物技术潜力:蛋白质组分析的贡献。
通过绿色合成路线合成的银纳米粒子(AgNPs)具有生物相容性、稳定性、可持续性、快速性和成本效益等优点,因此被广泛用作抗菌剂。虽然 AgNPs 似乎比银离子更有效,但其抗菌活性的相关机制尚未完全明了。迄今为止,最常见的 AgNPs 毒性机制是银离子的释放和/或颗粒的特定功能。在此背景下,本研究旨在通过蛋白质组学研究利用诺丽果皮(海巴戟)合成的 AgNPs 对植物病原体野油菜黄单胞菌(Xcc)的作用机制。用AgNPs(32 µM)、AgNO3(32 µM)或不处理(Ctrl - 对照条件)对Xcc进行处理,并对其蛋白质组学反应进行综合表征,以阐明AgNPs对植物病原微生物的抗菌机制。共鉴定出 352 个差异丰度蛋白。在 AgNPs × Ctrl 和 AgNPs × AgNO3 的比较/条件下,大多数蛋白质都受到了调控。将用 32 µM AgNPs 处理的 Xcc 与对照组进行比较,结果显示有 134 种蛋白质含量不同,其中 107 种蛋白质含量增加,27 种蛋白质含量减少。与此相反,当用 32 µM AgNO3 处理 Xcc 与对照组进行比较时,结果显示只有 14 个蛋白质含量不同,其中包括 10 个增加的蛋白质和 4 个减少的蛋白质。最后,当用 32 µM AgNPs 处理的 Xcc 与用 32 µM AgNO3 处理的 Xcc 相比时,结果显示出 204 个不同的丰富度蛋白,包括 75 个增加的蛋白和 129 个减少的蛋白。基因本体富集分析表明,大多数增加的蛋白质参与了重要的生物过程,如金属离子平衡、解毒、膜组织、氨基酸和碳水化合物代谢过程、脂代谢过程、蛋白质分解、跨膜运输等。本研究中使用的 AgNPs 对植物病原菌 Xcc 具有有效的抗菌活性。此外,研究结果还有助于更好地了解 AgNPs 对 Xcc 的作用机制,并有助于制定控制黄铜病菌 Xcc 的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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