Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Guilherme Caldieraro Viana, Leonardo Oliveira Médici, Marcia Soares Vidal, José Ivo Baldani
{"title":"Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha.","authors":"Guilherme Caldieraro Viana, Leonardo Oliveira Médici, Marcia Soares Vidal, José Ivo Baldani","doi":"10.1007/s42770-024-01539-z","DOIUrl":null,"url":null,"abstract":"<p><p>Fusarium wilt is one of main phytopathology attacking tomato (Solanum lycopersicum L.) plantations in Brazil. Plant rhizosphere and endophytic beneficial microorganism are well known as plant growth promoters and biocontrol agents. The present study aims to evaluate the potential of different Bacillus strains as biocontrol agent to Fusarium oxysporum f. sp. lycopersici Race 3 strains; and also as plant growth promoting bacteria on Solanum lycopersicum cv Perinha. Different in vitro and greenhouse experiments were carried out to evaluate the direct and indirect bacterial-fungus antagonism, and they inoculation effects on plant traits. In vitro direct, metabolites, and volatile antagonism analysis demonstrated that B. toyonensis BR 10491(FORT 02) presented a broad antagonism to all tested race 3 FOL strains while B. megaterium BR 10466 (FORT 12), B. aryabhattai BR 10494 (FORT 25), B. stratosphericus BR 10438 (FORT 29) and B. cereus BR 10493 (FORT 113.1) strains showed significant antagonistic activity for at least two applied methods. Greenhouse pot experiments demonstrated a significant BCA effect of FORT 113.1 and FORT 02 against FOL Race 3 Fus 1302 strain during different tomato development stages (seedling, vegetative, and reproductive). Bacillus cereus (FORT 113.1) showed significantly higher shoot and height fresh weight, Chlorophyll a and Chlorophyll b content, stomata conductance, water use efficiency, and also a lower xylem infection percentage during vegetative and reproductive stages. Antioxidant enzymatic components analysis demonstrated a synergic effect of Fusarium and Bacillus inoculation, leading to a higher superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activity. In conclusion, the results suggest that strain FORT113.1 could be considered as a good candidate for production of new biofungicide with high potential to augment the existing biocontrol strategies.</p>","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-024-01539-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Fusarium wilt is one of main phytopathology attacking tomato (Solanum lycopersicum L.) plantations in Brazil. Plant rhizosphere and endophytic beneficial microorganism are well known as plant growth promoters and biocontrol agents. The present study aims to evaluate the potential of different Bacillus strains as biocontrol agent to Fusarium oxysporum f. sp. lycopersici Race 3 strains; and also as plant growth promoting bacteria on Solanum lycopersicum cv Perinha. Different in vitro and greenhouse experiments were carried out to evaluate the direct and indirect bacterial-fungus antagonism, and they inoculation effects on plant traits. In vitro direct, metabolites, and volatile antagonism analysis demonstrated that B. toyonensis BR 10491(FORT 02) presented a broad antagonism to all tested race 3 FOL strains while B. megaterium BR 10466 (FORT 12), B. aryabhattai BR 10494 (FORT 25), B. stratosphericus BR 10438 (FORT 29) and B. cereus BR 10493 (FORT 113.1) strains showed significant antagonistic activity for at least two applied methods. Greenhouse pot experiments demonstrated a significant BCA effect of FORT 113.1 and FORT 02 against FOL Race 3 Fus 1302 strain during different tomato development stages (seedling, vegetative, and reproductive). Bacillus cereus (FORT 113.1) showed significantly higher shoot and height fresh weight, Chlorophyll a and Chlorophyll b content, stomata conductance, water use efficiency, and also a lower xylem infection percentage during vegetative and reproductive stages. Antioxidant enzymatic components analysis demonstrated a synergic effect of Fusarium and Bacillus inoculation, leading to a higher superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activity. In conclusion, the results suggest that strain FORT113.1 could be considered as a good candidate for production of new biofungicide with high potential to augment the existing biocontrol strategies.

芽孢杆菌内生菌株控制番茄变种 Fusarium oxysporum f. sp. lycopersici 引起的镰刀菌枯萎病。Perinha。
镰刀菌枯萎病是危害巴西番茄(Solanum lycopersicum L.)种植园的主要植物病理学之一。植物根瘤菌和内生有益微生物是众所周知的植物生长促进剂和生物控制剂。本研究旨在评估不同芽孢杆菌菌株作为 Fusarium oxysporum f. sp. lycopersici Race 3 菌株生物控制剂的潜力,以及作为植物生长促进菌对 Solanum lycopersicum cv Perinha 的影响。为了评估细菌与真菌的直接和间接拮抗作用,以及它们对植物性状的接种影响,进行了不同的体外和温室实验。体外直接拮抗、代谢物拮抗和挥发性拮抗分析表明,B. toyonensis BR 10491(FORT 02)对所有测试的第 3 种族 FOL 菌株具有广泛的拮抗作用,而 B. megaterium BR 10466(FORT 12)、B. aryabhattai BR 10494(FORT 25)、B. stratosphericus BR 10438(FORT 29)和 B. cereus BR 10493(FORT 113.1)菌株至少在两种应用方法中表现出显著的拮抗活性。温室盆栽实验表明,FORT 113.1 和 FORT 02 在番茄的不同发育阶段(幼苗期、无性繁殖期和生殖期)对 FOL Race 3 Fus 1302 菌株具有显著的 BCA 效果。蜡样芽孢杆菌(FORT 113.1)在无性和生殖阶段的嫩枝和高度鲜重、叶绿素 a 和叶绿素 b 含量、气孔导度、水分利用效率都明显较高,木质部感染率也较低。抗氧化酶成分分析表明,镰刀菌和芽孢杆菌接种会产生协同效应,导致更高的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性。总之,研究结果表明,菌株 FORT113.1 可被视为生产新型生物杀真菌剂的理想候选菌株,具有增强现有生物防治策略的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信