Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Molla Zakirul Haque, Chanchal Guha, Ayan Mukherjee, Sukhen Samanta, Partha Sarathi Jana, Ujjwal Biswas, Sangeeta Mandal, Santanu Pal, Manigandan Venkatesan, Joy Sarojini Michael, Pramod Kumar Nanda, Samiran Bandyopadhyay, Arun K Das, Premanshu Dandapat
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Abstract

Background: Tuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bTB) a cumbersome task. Therefore, a cross sectional study was conducted to isolate and characterize different Mycobacterium spp. from a slaughterhouse situated in Kolkata, a city in the eastern part of India.

Results: Out of 258 morbid samples, 98 isolates were found to be positive for bacterial growth, and 35% (n = 34) were positive for Mycobacterium. 94% of Mycobacterial cultural isolates were NTM (n = 32), and the rest (n = 2) were found to be MTBC. Species-level identification of the isolates by hsp65 sequencing revealed that out of 32 isolates, 24 were M. fortuitum, three were M. abscessus, two each were M. chelonae and M. parascrofulaceum, and one was M. novocastrense. A phylogenetic tree with partial hsp65 gene sequences was also constructed to determine the relatedness of the unknown isolates to the reference strains.

Conclusion: Both NTM species and MTBCs were identified from TB-like lesions in cattle that were slaughtered at the Kolkata abattoir. This discovery may indicate that NTM contributes to the development of lesions in cattle. Also, we recommend implication of more specific diagnostic tests for bTB.

通过监测加尔各答屠宰牛中的分枝杆菌种类并确定其特征来诊断牛结核病所面临的挑战。
背景:牛结核病是由复合结核分枝杆菌(MTBC)引起的。除 MTBC 外,牛体内还分离出不同的非结核分枝杆菌 (NTM)。牛感染非结核分枝杆菌使得牛结核病(bTB)的诊断变得十分棘手。因此,我们开展了一项横断面研究,从印度东部城市加尔各答的一个屠宰场中分离出不同的分枝杆菌属并确定其特征:结果:在 258 个病死样本中,发现 98 个分离物的细菌生长呈阳性,35%(n = 34)对分枝杆菌呈阳性。94%的分枝杆菌培养分离物为 NTM(n = 32),其余(n = 2)为 MTBC。通过 hsp65 测序对分离株进行种级鉴定发现,32 个分离株中有 24 个是 M. fortuitum,3 个是 M. abscessus,M. chelonae 和 M. parascrofulaceum 各有 2 个,还有 1 个是 M. novocastrense。还利用部分 hsp65 基因序列构建了系统发生树,以确定未知分离株与参考菌株的亲缘关系:结论:在加尔各答屠宰场屠宰的牛的结核病样病变中发现了 NTM 和 MTBC。这一发现可能表明,NTM 是导致牛发生病变的原因之一。此外,我们还建议对结核病进行更具针对性的诊断测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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