Quantifying skin permeation of a novel metformin lotion using modified hydrophilic interaction liquid chromatography.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-01-01 Epub Date: 2024-10-16 DOI:10.1080/17576180.2024.2412438

Derek J Maloney, Jianying Zhang, Satyaj Bhargava, MaCalus V Hogan, James H-C Wang

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Abstract

Aim: This study aimed to quantify the permeation of metformin (Met) lotion through pig ear skin using high-performance liquid chromatography, specifically hydrophilic interaction liquid chromatography (HILIC), to separate Met from biological contaminants and effectively measure its permeation through skin similar to human skin.Materials & methods: A Franz cell permeation assay was used to assess the permeation kinetics of 6% Met lotion through pig ear skin. Samples were collected at various time points and prepared for high-performance liquid chromatography analysis by removing large biological contaminants. The permeated Met was quantified by monitoring its retention time (RT) at 9 min using HILIC, with an acidic, polar mobile phase and a normal-phase column.Results: A distinct Met peak with a RT of approximately 9 min was observed in the 6% Met lotion, which was absent in the permeation samples from the 0% Met lotion. This peak (RT 9 min) was distinct from the 'biological-contaminants' peaks at RT 2-3 min and increased linearly over time, reaching 36.8% of the total applied Met at 24 h.Conclusion: These findings demonstrate that the HILIC method effectively separates Met from biological components in pig ear skin, allowing accurate quantification of Met despite the presence of skin lipids and proteins.

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利用改良亲水相互作用液相色谱法量化新型二甲双胍乳液的皮肤渗透性。

目的：本研究旨在利用高效液相色谱法，特别是亲水作用液相色谱法（HILIC），定量测定二甲双胍（Met）乳液在猪耳皮肤中的渗透情况，从而将二甲双胍从生物污染物中分离出来，并有效测定其在与人类皮肤相似的皮肤中的渗透情况：采用弗朗兹细胞渗透试验来评估 6% Met 洗剂在猪耳皮肤中的渗透动力学。在不同时间点采集样品，去除大量生物杂质后备用于高效液相色谱分析。采用酸性极性流动相和正相色谱柱，使用 HILIC 监测 9 分钟的保留时间（RT），对渗透的 Met 进行定量：结果：在 6% 金属元素乳液中观察到了一个明显的金属元素峰，保留时间约为 9 分钟，而在 0% 金属元素乳液的渗透样品中则没有这个峰。该峰值（RT 9 分钟）与 "生物污染物 "峰值（RT 2-3 分钟）截然不同，并且随着时间的推移呈线性增长，在 24 小时后达到所应用 Met 总量的 36.8%：这些研究结果表明，HILIC 方法能有效地将金属元素与猪耳皮肤中的生物成分分离开来，即使皮肤中存在脂质和蛋白质，也能准确定量金属元素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.